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PMID: 6709503 Published · ppublish English Journal Article

Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.

Nucleic acids research ·Vol. 12 ·No. 6 ·1984-03-26 ·Pages 2823-36

Sanford J, Forrester L, Chapman V, Chandley A, Hastie N

Abstract

The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic DNA methylation between sperm and oocyte DNA. The methylation levels of the minor satellite sequences did not change during spermiogenesis, and were not associated with the onset of meiosis or a specific stage in sperm development.

MeSH Terms
Animals Base Composition Cloning, Molecular DNA, Satellite/isolation & purification Female Male Methylation Mice Mice, Inbred Strains Molecular Weight Oocytes/analysis Plasmids Repetitive Sequences, Nucleic Acid Spermatozoa/analysis
Chemicals
DNA, Satellite
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sanford J
Forrester L
Chapman V
Chandley A
Hastie N
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.6.2823
Published
1984-03-26
Pages
2823-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318708
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.6.2823 PMC Full Text View journal details
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