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PMID: 6272210 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues.

Nucleic acids research ·Vol. 9 ·No. 18 ·1981-09-25 ·Pages 4537-46

Sturm KS, Taylor JH

Abstract

Genomic DNA of calf thymus contains 1.5 times as much 5-methylcytosine as similar sperm DNA, but the major EcoRI repeat fragment from satellite I of thymus contains ten times as much 5-methylcytosine as the corresponding fragment from sperm DNA. Restriction enzyme analyses of the total DNA and the satellite I fragment show that three HpaII sites in the fragment are completely unmethylated in sperm but fully methylated in thymus DNA. Under-methylation of many sites in the satellite DNAs can probably account for the lower level of methylation of sperm DNA rather than hemimethylation as previously suggested. These results are also discussed in relation to maintenance and de novo (initiation-type) methylases.

MeSH Terms
5-Methylcytosine Animals Cattle Cytosine/analogs & derivatives,analysis DNA/analysis DNA Restriction Enzymes Genes Male Organ Specificity Spermatozoa/analysis Thymus Gland/analysis
Chemicals
5-Methylcytosine Cytosine DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sturm K S
Taylor J H
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.18.4537
Published
1981-09-25
Pages
4537-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327456
Subset
IM
Grants
NIA NIH HHS · R01 AG01807-01 · United States
External Links
PubMed source DOI: 10.1093/nar/9.18.4537 PMC Full Text View journal details
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