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PMID: 523314 Published · ppublish English Journal Article

DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.

Nucleic acids research ·Vol. 7 ·No. 8 ·1979-12-20 ·Pages 2081-103

Mandel JL, Chambon P

Abstract

The restriction enzymes HhaI and HpaII, whose activity is inhibited by cytosine methylation within their recognition sites, have been utilised as probes to study methylation in the vicinity of the ovalbumin gene in DNA from various chicken tissues. This was complemented by a preliminary study of methylation in the regions of chicken ovotransferrin (conalbumin), ovomucoid and beta-globin genes. From our data we conclude that HaI or HpaII sites can be divided in 3 classes according to their pattern of methylation in different tissues. In the first class of sites (mV class) the extent of methylation varies in different tissues. The patterns obtained show that methylation at the sites located within and around the 3 genes which code for egg white proteins is in general lowest in oviduct of laying hen, where these genes are expressed. However some sites are not methylated (m- class) and others are 95 to 100% resistant (m+ class) to digestion by HhaI or HpaII in the DNAs of all the tissues which were tested. Our study has also revealed a remarkable number of allelic variants for the presence of HhaI or HpaII sites in the region of the ovalbumin gene.

MeSH Terms
Animals Chickens Conalbumin/biosynthesis DNA DNA Restriction Enzymes Egg Proteins/biosynthesis Female Genes Homozygote Male Methylation Ovalbumin/biosynthesis Oviducts/analysis Tissue Distribution
Chemicals
Egg Proteins Conalbumin Ovalbumin DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mandel J L
Chambon P
References (43)
43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.8.2081
Published
1979-12-20
Pages
2081-103
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342372
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.8.2081 PMC Full Text View journal details
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