Methylation of DNA in early development: 5-methyl cytosine content of DNA in sea urchin sperm and embryos.

Nucleic acids research ·Vol. 5 ·No. 12 ·1978-12-00 ·Pages 4855-61

Pollock JM, Swihart M, Taylor JH

Abstract

By separating formic acid hydrolysates with high pressure chromatography on an Aminex-10 column, we determined the ratio of 5-methyl cytosine to cytosine and other bases of DNA from sea urchin sperm and nuclei of embryos from early cleavage through pluteus stages. Contrary to several previous reports, we could not find any measurable changes in the methylation levels of embryonic nuclear DNAs at different stages of development. We also found no consistent differences between the methylation levels of sea urchin sperm and embryonic nuclei or the 5-methyl cytosine content of fish (Mugil cephalus) sperm and liver nuclei. While these measurements would not have detected subtle variations associated with differentiation, they would have indicated the gross changes previously reported for embryos or between sperm and somatic nuclei had those changes been present.

MeSH Terms

Animals Cytosine/analogs & derivatives,analysis DNA/metabolism Embryo, Nonmammalian/metabolism Female Fishes Liver/analysis Male Methylation Sea Urchins Spermatozoa/metabolism

Chemicals

Cytosine DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pollock J M

Swihart M

Taylor J H

References (19)

19 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.12.4855

Published

1978-12-00

Pages

4855-61

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342793

Subset

External Links

PubMed source DOI: 10.1093/nar/5.12.4855 PMC Full Text View journal details

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Methylation of DNA in early development: 5-methyl cytosine content of DNA in sea urchin sperm and embryos.

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