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PMID: 4631666 Published · ppublish English Journal Article

( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.

The Journal of cell biology ·Vol. 56 ·No. 3 ·1973-03-00 ·Pages 697-701

Gorovsky MA, Hattman S, Pleger GL

Abstract

DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65-0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).

MeSH Terms
Adenine/analysis Animals Cell Nucleus/analysis DNA/analysis Germ-Free Life Methylation Tetrahymena pyriformis/analysis,cytology Tritium
Chemicals
Tritium DNA Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gorovsky M A
Hattman S
Pleger G L
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21 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.56.3.697
Published
1973-03-00
Pages
697-701
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108936
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.56.3.697 PMC Full Text View journal details
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