Molecular basis of base substitution hotspots in Escherichia coli.

Nature ·Vol. 274 ·No. 5673 ·1978-08-24 ·Pages 775-80

Coulondre C, Miller JH, Farabaugh PJ, Gilbert W

Abstract

In the lacI gene of Escherichia coli spontaneous base substituion hotspots occur at 5-methylcytosine residues. The hotspots disappear when the respective cytosines are not methylated. We suggest that the hotspots may result from the spontaneous deamination of 5-methylcytosine to thymine, which is not excised by the enzyme DNA-uracil glycosidase.

MeSH Terms

2-Aminopurine/pharmacology Base Sequence Codon Cytosine/analogs & derivatives Escherichia coli/genetics Genes Lactose/genetics Mutagens Mutation/drug effects,radiation effects Peptide Chain Termination, Translational Ultraviolet Rays

Chemicals

Codon Mutagens 2-Aminopurine Cytosine Lactose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Coulondre C

Miller J H

Farabaugh P J

Gilbert W

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/274775a0

Published

1978-08-24

Pages

775-80

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/274775a0 View journal details

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Molecular basis of base substitution hotspots in Escherichia coli.

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