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PMID: 283395 Published · ppublish English Journal Article

Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4853-7

Breathnach R, Benoist C, O'Hare K, Gannon F, Chambon P

Abstract

Selected regions of cloned EcoRI fragments of the chicken ovalbumin gene have been sequenced. The positions where the sequences coding for ovalbumin mRNA (ov-mRNA) are interrupted in the genome have been determined, and a previously unreported interruption in the DNA sequences coding for the 5' nontranslated region of the messenger has been discovered. Because directly repeated sequences are found at exon-intron boundaries, the nucleotide sequence alone cannot define unique excision-ligation points for the processing of a possible ov-mRNA precursor. However, the sequences in these boundary regions share common features; this leads to the proposal that there are, in fact, unique excision-ligation points common to all boundaries.

MeSH Terms
Animals Base Sequence Chickens/genetics DNA Genes Ovalbumin/genetics RNA, Messenger
Chemicals
RNA, Messenger Ovalbumin DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Breathnach R
Benoist C
O'Hare K
Gannon F
Chambon P
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4853
Published
1978-10-00
Pages
4853-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336219
Subset
IM
Databases
GENBANK
J00895
External Links
PubMed source DOI: 10.1073/pnas.75.10.4853 PMC Full Text View journal details
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