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PMID: 600794 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Specificity of Hpa II and Hae III DNA methylases.

Nucleic acids research ·Vol. 4 ·No. 12 ·1977-12-00 ·Pages 4211-21

Mann MB, Smith HO

Abstract

The methylases M.HaeIII and M.HpaII recognize the tetranucleotide sequences [Formula: see text] and [Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R.HaeIII does not cleave the methylated sequence [Formula: see text] but can cleave [Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R.HpaII does not cleave [Formula: see text] but can cleave [Formula: see text].Images

MeSH Terms

Base Sequence DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Haemophilus/enzymology Methyltransferases Oligodeoxyribonucleotides/analysis Species Specificity Substrate Specificity

Chemicals

Oligodeoxyribonucleotides Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mann M B

Smith H O

References (11)

11 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.12.4211

Published

1977-12-00

Pages

4211-21

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343235

Subset

External Links

PubMed source DOI: 10.1093/nar/4.12.4211 PMC Full Text View journal details

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