Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

Nucleic acids research ·Vol. 4 ·No. 2 ·1977-02-00 ·Pages 353-71

Stawinski J, Hozumi T, Narang SA, Bahl CP, Wu R

Abstract

The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.

MeSH Terms

DNA Restriction Enzymes Indicators and Reagents Methods Oligodeoxyribonucleotides/chemical synthesis,isolation & purification Oligonucleotides/chemical synthesis Phosphoric Diester Hydrolases Tetrazoles

Chemicals

Indicators and Reagents Oligodeoxyribonucleotides Oligonucleotides Tetrazoles DNA Restriction Enzymes Phosphoric Diester Hydrolases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stawinski J

Hozumi T

Narang S A

Bahl C P

Wu R

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.2.353

Published

1977-02-00

Pages

353-71

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342437

Subset

External Links

PubMed source DOI: 10.1093/nar/4.2.353 PMC Full Text View journal details

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Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.

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