DNA-methylase from regenerating rat liver: purification and characterisation.

Nucleic acids research ·Vol. 5 ·No. 6 ·1978-06-00 ·Pages 2153-67

Simon D, Grunert F, von Acken U, Döring HP, Kröger H

Abstract

DNA methylase has been purified 660-fold from nuclei from regenerating rat liver. The enzyme is able to methylate single stranded (ss) and double stranded (ds) DNA, the only reaction product being 5-methylcytosine. Previously unmethylated double stranded DNA from prokaryotes (M.luteus) as well as from eukaryotes (Ascaris suis) can serve as substrates. The synthetic copolymers (dG-dC)n . (dC-dG)n and (dG,dC)n are also methylated. While SV40 DNA is almost not methylated, PM2 DNA is a good substrate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous M.luteus DNA, but only 1 in 590 in homologous rat liver DNA. The high methylation level of M.luteus DNA, an analysis of the methylated pyrimidine isostichs and a preliminary dinucleotide analysis suggest that all the CpGs in a DNA can be methylated.

MeSH Terms

Animals DNA (Cytosine-5-)-Methyltransferases/isolation & purification,metabolism Kinetics Liver/enzymology Liver Regeneration Male Methyltransferases/metabolism Osmolar Concentration Rats Substrate Specificity

Chemicals

Methyltransferases DNA (Cytosine-5-)-Methyltransferases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Simon D

Grunert F

von Acken U

Döring H P

Kröger H

References (28)

28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.6.2153

Published

1978-06-00

Pages

2153-67

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342150

Subset

External Links

PubMed source DOI: 10.1093/nar/5.6.2153 PMC Full Text View journal details

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DNA-methylase from regenerating rat liver: purification and characterisation.

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