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PMID: 6253937 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Variable patterns of total DNA and rDNA methylation in animals.

Nucleic acids research ·Vol. 8 ·No. 7 ·1980-04-11 ·Pages 1485-97

Bird AP, Taggart MH

Abstract

Restriction endonucleases were used to determine the degree of methylation at the sequences CCGG and GCGC in a wide range of animal DNAs. Both total DNA methylation and ribosomal DNA methylation were studied. Whole DNA methylation was indetectable in arthropods, fractional in other invertebrate phyla, and high in the vertebrates. Ribosomal DNA was predominantly unmethylated in all animals except fish and amphibia, where it was heavily methylated. We discuss the evolutionary and functional implications of these results, and suggest that the large differences between genome types are the result of evolutionary changes in the relative size of heavily methylated and unmethylated compartments.

MeSH Terms
Animals Base Sequence DNA/analysis DNA Restriction Enzymes Female Humans Invertebrates Male Methylation Molecular Weight Nucleic Acid Hybridization Pregnancy Ribosomes/analysis Species Specificity Vertebrates
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bird A P
Taggart M H
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.7.1485
Published
1980-04-11
Pages
1485-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324011
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.7.1485 PMC Full Text View journal details
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