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PMID: 523319 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Methylation of somatic vs germ cell DNAs analyzed by restriction endonuclease digestions.

Nucleic acids research ·Vol. 7 ·No. 8 ·1979-12-20 ·Pages 2303-22

Kaput J, Sneider TW

Abstract

Bacterial restriction endonucleases containing the dinucleotide CpG in their cleavage sequences were used to compare the methylation patterns of primarily repeated DNA sequences in (1) bovine somatic cell native DNAs vs bovine sperm cell native DNA and (2) native vs renatured bovine liver and sperm cell DNAs. The restriction patterns of sperm native DNA differ markedly from those of somatic cell native DNAs when using Hpa II, Hha I, and Ava I but not when using the enzymes Eco RI and Msp I. Digestion patterns of germ cell renatured DNA differed significantly from those of germ cell native DNA when using Hpa II but not when using Msp I or Eco RI. The results may not be due to artifacts of renaturation of the DNAs. The results are consistent with the concept that germ cell DNA may be strand asymmetrically hemimethylated. The data also suggest that methylation of the 5'-cytosine in the sequence CCGG renders this site insensitive to cleavage by Msp I.

MeSH Terms
Animals Base Sequence Cattle DNA/isolation & purification DNA Restriction Enzymes Kidney/analysis Liver/analysis Liver Neoplasms, Experimental/analysis Male Methylation Nucleic Acid Denaturation Organ Specificity Rats Spermatozoa/analysis Thymus Gland/analysis
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaput J
Sneider T W
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.8.2303
Published
1979-12-20
Pages
2303-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342386
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.8.2303 PMC Full Text View journal details
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