Evidence for DNA modification in the maintenance of X-chromosome inactivation of adult mouse tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5357-61

Chapman VM, Kratzer PG, Siracusa LD, Quarantillo BA, Evans R, Liskay RM

Abstract

The role of DNA modification in the maintenance of mammalian X-chromosome inactivation was investigated by using the technique of DNA transformation in mammalian cells. The ability of inactive X-chromosome DNA from adult mouse tissues to act in transformation for the X-linked hypoxanthine phosphoribosyltransferase gene (Hprt) could be ascertained by utilizing a recently discovered electrophoretic variant form of the hypoxanthine phosphoribosyltransferase enzyme and a previously available X:autosome translocation. Our findings indicate that inactive X-chromosome DNA from several tissues of adult female mice is strikingly inefficient, in comparison to active X-chromosome DNA, in eliciting genetic transformation for hypoxanthine phosphoribosyltransferase. These results provide in vivo evidence that is consistent with DNA modification playing an important role in the maintenance of X-chromosome inactivation.

MeSH Terms

Animals Bone Marrow/physiology DNA/genetics Female Genes Hypoxanthine Phosphoribosyltransferase/genetics Isoelectric Focusing Male Mice Phenotype Sex Chromosomes/physiology Translocation, Genetic X Chromosome/physiology

Chemicals

DNA Hypoxanthine Phosphoribosyltransferase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Chapman V M

Kratzer P G

Siracusa L D

Quarantillo B A

Evans R

Liskay R M

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.17.5357

Published

1982-09-00

Pages

5357-61

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC346895

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.17.5357 PMC Full Text View journal details

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Evidence for DNA modification in the maintenance of X-chromosome inactivation of adult mouse tissues.

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