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PMID: 6196723 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Methylation of unique sequence DNA during spermatogenesis in mice.

Nucleic acids research ·Vol. 11 ·No. 22 ·1983-11-25 ·Pages 7947-59

Rahe B, Erickson RP, Quinto M

Abstract

In order to study whether changes in methylation of unique sequence DNA were related to meiosis, DNA was purified from F9 embryonal carcinoma (a "primordial germ cell" equivalent), germ cells from immature testes (containing germ cells up to early spermatocytes), sperm, and appropriate somatic tissues. Restriction was performed with the isoschizomers Msp I and Hpa II, and Eco RI as a control. Electrophoresis and Southern transfers were followed by hybridization to a mouse major beta-globin clone (f7), a mouse pancreatic amylase clone (pMPa21), a type I, histocompatibility-2 clone (pH-2D-4), and a spermatid cDNA clone (pPM 459). The variably methylated sites were all hypomethylated in the embryonal carcinoma DNA and hypermethylated in DNA from immature testes and sperm, irrespective of the transcription state of the gene. The pattern in control tissues generally conformed to an inverse correlation of methylation with transcription. These results suggest that hypermethylation of sperm DNA persists from hypermethylation of these sequences early in testicular development, independent of gene expression.

MeSH Terms
Amylases/genetics Animals Base Sequence Cloning, Molecular DNA/analysis,metabolism Globins/genetics H-2 Antigens/genetics Male Meiosis Methylation Mice Mice, Inbred C57BL Nucleic Acid Hybridization Pancreas/enzymology Spermatogenesis Spermatozoa/physiology Testis/physiology
Chemicals
H-2 Antigens Globins DNA Amylases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rahe B
Erickson R P
Quinto M
References (43)
43 references, click to expand
  1. Methylation of simian virus 40 Hpa II site affects late, but not early, viral gene expression.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5142-6 PMID: 6291026
  2. Undermethylation at the 5' end of the albumin gene is necessary but not sufficient for albumin production by rat hepatoma cells in culture.
    Cell. 1982 Oct;30(3):825-33 PMID: 7139715
  3. DNA methylation patterns of human pachytene spermatocytes.
    Exp Cell Res. 1982 Oct;141(2):253-9 PMID: 6754392
  4. Functional assays for mRNA detect many new messages after male meiosis in mice.
    Biochem Biophys Res Commun. 1982 Oct 29;108(4):1369-75 PMID: 6129844
  5. In vitro methylation of specific regions of the cloned Moloney sarcoma virus genome inhibits its transforming activity.
    Mol Cell Biol. 1983 Mar;3(3):305-14 PMID: 6843547
  6. Pattern of methylation of two genes coding for housekeeping functions.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2422-6 PMID: 6302681
  7. Distribution of 5-methylcytosine in pyrimidine sequences of deoxyribonucleic acids.
    Biochim Biophys Acta. 1962 Jun 11;55:953-9 PMID: 13887466
  8. Rare bases in animal DNA.
    Nature. 1970 Mar 7;225(5236):948-9 PMID: 4391887
  9. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  10. Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988-92 PMID: 4355379
  11. Letter: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture.
    Transplantation. 1974 Jun;17(6):632-4 PMID: 4829671
  12. DNA modification mechanisms and gene activity during development.
    Science. 1975 Jan 24;187(4173):226-32 PMID: 1111098
  13. X inactivation, differentiation, and DNA methylation.
    Cytogenet Cell Genet. 1975;14(1):9-25 PMID: 1093816
  14. Absence of H-2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3661-5 PMID: 52873
  15. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  16. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  17. Specificity of Hpa II and Hae III DNA methylases.
    Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
  18. F9 embryonal carcinoma cells can differentiate into endoderm-like cells.
    Dev Biol. 1978 Mar;63(1):27-34 PMID: 631430
  19. The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
    Cell. 1978 Oct;15(2):393-403 PMID: 214238
  20. DNA methylation at a CCGG sequence in the large intron of the rabbit beta-globin gene: tissue-specific variations.
    Nucleic Acids Res. 1978 Dec;5(12):4631-4 PMID: 745990
  21. Specific DNA methylation sites in the vicinity of the chicken beta-globin genes.
    Nature. 1979 Aug 2;280(5721):419-20 PMID: 460418
  22. Methylation of Herpesvirus saimiri DNA in lymphoid tumor cell lines.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3839-43 PMID: 226983
  23. Isolation and characterization of germ line DNA from mouse sperm.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3947-51 PMID: 291053
  24. DNA methylation: organ specific variations in the methylation pattern within and around ovalbumin and other chicken genes.
    Nucleic Acids Res. 1979 Dec 20;7(8):2081-103 PMID: 523314
  25. Methylation of somatic vs germ cell DNAs analyzed by restriction endonuclease digestions.
    Nucleic Acids Res. 1979 Dec 20;7(8):2303-22 PMID: 523319
  26. Methylation of DNA in mouse early embryos, teratocarcinoma cells and adult tissues of mouse and rabbit.
    Nucleic Acids Res. 1979 Dec 20;7(8):2369-85 PMID: 523320
  27. DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.
    Cell. 1980 Apr;19(4):947-58 PMID: 6247075
  28. DNA sequence organization of the beta-globin complex in the BALB/c mouse.
    Cell. 1980 Aug;21(1):159-68 PMID: 6250710
  29. Tissue-specific expression of mouse alpha-amylase genes.
    J Mol Biol. 1980 Sep 5;142(1):93-116 PMID: 6159478
  30. The 5'-cytosine in CCGG1 is methylated in two eukaryotic DNAs and Msp I is sensitive to methylation at this site.
    Nucleic Acids Res. 1980 Sep 11;8(17):3829-40 PMID: 6255419
  31. Tissue-specific DNA methylation in a cluster of rabbit beta-like globin genes.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6634-8 PMID: 6935674
  32. Active gene sequences are undermethylated.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4246-50 PMID: 6945582
  33. Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues.
    Nucleic Acids Res. 1981 Sep 25;9(18):4537-46 PMID: 6272210
  34. Expression of the J chain gene during B cell differentiation is inversely correlated with DNA methylation.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4907-11 PMID: 6795624
  35. DNA methylation--a regulatory signal in eukaryotic gene expression.
    J Gen Virol. 1981 Nov;57(Pt 1):1-20 PMID: 6275010
  36. Tissue specificity and organisation of CpG methylation in calf satellite DNA I.
    Nucleic Acids Res. 1982 Jan 22;10(2):565-76 PMID: 6278421
  37. Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.
    Nucleic Acids Res. 1982 Feb 11;10(3):1039-49 PMID: 6278432
  38. Immunoglobulin heavy chain genes: demethylation accompanies class switching.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7497-501 PMID: 6801653
  39. Expression of a cloned adenovirus gene is inhibited by in vitro methylation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1073-7 PMID: 6951163
  40. Amount and distribution of 5-methylcytosine in human DNA from different types of tissues of cells.
    Nucleic Acids Res. 1982 Apr 24;10(8):2709-21 PMID: 7079182
  41. Major histocompatibility antigens are not expressed on human epididymal sperm.
    J Immunol. 1982 Aug;129(2):452-4 PMID: 6806369
  42. Variability of DNA methylation patterns during serial passage of human diploid fibroblasts.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):3949-53 PMID: 6287455
  43. Alpha-Globin-gene switching during the development of chicken embryos: expression and chromosome structure.
    Cell. 1981 May;24(2):333-44 PMID: 7237551
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.22.7947
Published
1983-11-25
Pages
7947-59
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326551
Subset
IM
Grants
NICHD NIH HHS · HD 11738 · United States
External Links
PubMed source DOI: 10.1093/nar/11.22.7947 PMC Full Text View journal details
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