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PMID: 6278421 Published · ppublish English Journal Article

Tissue specificity and organisation of CpG methylation in calf satellite DNA I.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 565-76

Pages M, Roizes G

Abstract

Examination of bovine satellite DNA I methylation within CpG dinucleotides has been made by restriction analysis. It is shown that variations in the methylation patterns occur between different tissues (brain, liver, thymus and sperm) . Some of the 8 Hpa II sites present per repeat are clearly undermethylated in sperm as compared to other tissues. Methylation is considered therefore, as a highly specific event. It is also shown that there is a spatial specificity in the methylation pattern of the 3 Hha I sites in all tissues. These results are discussed in the light of methylation and satellite DNA functions.

MeSH Terms
Animals Brain Chemistry Cattle Cytidine Monophosphate/analogs & derivatives,analysis Cytosine Nucleotides/analysis DNA Restriction Enzymes DNA, Satellite/isolation & purification Dinucleoside Phosphates Guanosine/analogs & derivatives,analysis Liver/analysis Male Methylation Repetitive Sequences, Nucleic Acid Spermatozoa/analysis Thymus Gland/analysis
Chemicals
Cytosine Nucleotides DNA, Satellite Dinucleoside Phosphates Guanosine cytidylyl-3'-5'-guanosine DNA Restriction Enzymes Cytidine Monophosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pages M
Roizes G
References (40)
40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.565
Published
1982-01-22
Pages
565-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326158
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.2.565 PMC Full Text View journal details
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