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PMID: 6258142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Non-random arrangement of nucleosomes in satellite I containing chromatin of rat liver.

Nucleic acids research ·Vol. 8 ·No. 22 ·1980-11-25 ·Pages 5377-90

Igo-Kemenes T, Omori A, Zachau HG

Abstract

The location of nucleosomes on the nucleotide sequence of rat satellite I DNA was investigated using micrococcal nuclease, exonuclease III, and restriction nucleases as tools. Hae III cleaved the satellite DNA containing chromatin very preferentially in the linker region. Nucleosomes were found predominantly in three defined positions on the 370 bp satellite I monomer unit. This type of arrangement occurs on not more than half of the satellite DNA containing chromatin while the rest of this chromatin is arranged differently. The arrangement of nucleosomes with high probability in preferred frames and with low probability in less preferred frames may be a general phenomenon which can be discussed as a possible mechanism to modulate sequence recognition.

MeSH Terms

Animals Base Sequence Chromatin/analysis DNA Restriction Enzymes/metabolism DNA, Satellite/analysis Deoxyribonucleases/metabolism Deoxyribonucleases, Type II Site-Specific Liver/analysis Molecular Weight Nucleic Acid Hybridization Nucleosomes/analysis Rats

Chemicals

Chromatin DNA, Satellite Nucleosomes Deoxyribonucleases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GGCC-specific type II deoxyribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Igo-Kemenes T

Omori A

Zachau H G

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27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.22.5377

Published

1980-11-25

Pages

5377-90

Language

English

Region

England

NLM ID

0411011

PMCID

PMC324308

Subset

External Links

PubMed source DOI: 10.1093/nar/8.22.5377 PMC Full Text View journal details

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Non-random arrangement of nucleosomes in satellite I containing chromatin of rat liver.

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