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PMID: 523320 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Methylation of DNA in mouse early embryos, teratocarcinoma cells and adult tissues of mouse and rabbit.

Nucleic acids research ·Vol. 7 ·No. 8 ·1979-12-20 ·Pages 2369-85

Singer J, Roberts-Ems J, Luthardt FW, Riggs AD

Abstract

The distribution and amount of 5-methylcytosine (5-MeCyt) in DNA was measured for early embryos of mouse strain CF1 (2 to 4 cell stage to blastocyst) and mouse teratocarcinoma cells. In each case, the pattern of methylation was examined by use of the restriction enzymes Hha I and HPA II HPA II, which cut DNA at the sites 5'GCGC and 5'CCGG respectively, when the cytosines at these sites are not methylated. Mouse embryo DNA was found to have the same level of methylation as adult mouse tissues, and no changes in methylation were seen during differentiation of the teratocarcinoma cells. The ratio of 5-MeCyt/Cyt in DNA was measured by high performance liquid chromatography for the differentiating teratocarcinoma cells and for several adult mouse and rabbit tissues. The variation between tissues or between teratocarcinoma cells at different stages of differentiation was less than 10 percent. These results are discussed in view of proposals that 5-MeCyt plays a role in differentiation.

MeSH Terms
Animals Base Sequence Blastocyst Cytosine/analogs & derivatives,analysis DNA DNA, Neoplasm Embryo, Mammalian Female Male Methylation Mice Neoplasms, Experimental/analysis Pregnancy Rabbits Sex Factors Species Specificity Teratoma/analysis Tissue Distribution
Chemicals
DNA, Neoplasm Cytosine DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Singer J
Roberts-Ems J
Luthardt F W
Riggs A D
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.8.2369
Published
1979-12-20
Pages
2369-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342390
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.8.2369 PMC Full Text View journal details
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