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PMID: 9622 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.

Nucleic acids research ·Vol. 3 ·No. 9 ·1976-09-00 ·Pages 2183-91

Lin S, Lin D, Riggs AD

Abstract

Using a membrane filter assay, we have obtained results from both kinetic and competition experiments indicating that histones bind more strongly to bromodeoxyuridine-substituted DNA than to normal DNA. At 37 degrees C in our standard buffer of 0.2 M ionic strength, the rate of dissociation of histones H1, H2, and h4 from BrdU-substituted DNA is respectively 7, 4, and 2 times slower than it is from normal DNA. Competition experiments show an even greater difference between BrdU-substituted and normal DNA with respect to histone binding. The tighter binding of histones to BrdU-substituted DNA is of interest because of the known effects of BrdU on eukaryotic chromosome condensation and staining, virus induction, and the inhibition of differentiation.

MeSH Terms

Binding Sites Bromodeoxyuridine DNA DNA, Viral Filtration Histones Hydrogen-Ion Concentration Osmolar Concentration Plants Protein Binding

Chemicals

DNA, Viral Histones DNA Bromodeoxyuridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lin S

Lin D

Riggs A D

References (28)

28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.9.2183

Published

1976-09-00

Pages

2183-91

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343075

Subset

External Links

PubMed source DOI: 10.1093/nar/3.9.2183 PMC Full Text View journal details

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Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.

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