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PMID: 6856456 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The 5-methylcytosine content of highly repeated sequences in human DNA.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3087-95

Gama-Sosa MA, Wang RY, Kuo KC, Gehrke CW, Ehrlich M

Abstract

Previously, we found much tissue- or cell-specificity in the levels of 5-methylcytosine (m5C) in the total human genome as well as in DNA fractions resolved by reassociation kinetics. We now report that there were even greater differences in the m5C content of the highly repeated, tandem EcoRI family of DNA sequences from different human organs or cell populations. The ratio of m5C levels in this DNA fraction from brain, placenta, and sperm was 2.0:1.2:1.0. At a HhaI site in this repeat family, sperm DNA was 5-10 fold less methylated than somatic DNAs. In contrast, the highly repeated Alu family, which is approximately 5% of the genome, had almost the same high m5C content in brain and placenta despite marked tissue-specific differences in m5C levels of the single copy sequences with which these repeats are interspersed. These data show that very different degrees of change in methylation levels of various highly repeated DNA sequences accompany differentiation.

MeSH Terms
5-Methylcytosine Cytosine/analogs & derivatives,analysis DNA/isolation & purification Female Genes Humans Kinetics Male Methylation Nucleic Acid Hybridization Placenta/analysis Pregnancy Repetitive Sequences, Nucleic Acid Spermatozoa/analysis
Chemicals
5-Methylcytosine Cytosine DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gama-Sosa M A
Wang R Y
Kuo K C
Gehrke C W
Ehrlich M
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3087
Published
1983-05-25
Pages
3087-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325950
Subset
IM
Grants
NCI NIH HHS · CA-19942 · United States
External Links
PubMed source DOI: 10.1093/nar/11.10.3087 PMC Full Text View journal details
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