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PMID: 794832 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Repeating restriction fragments of human DNA.

Nucleic acids research ·Vol. 3 ·No. 11 ·1976-11-00 ·Pages 3063-76

Manuelidis L

Abstract

Human DNA digested with Hae III showed multiple repeats of a 170 base pair fragment. The most prominent band was the 340 base pair dimer, estimated to be 0.8% of the entire genome. Eco R1 and Hha I yielded fragments with similar electrophoretic mobility to the Hae III dimer. In each case this band was markedly enriched in DNA reassociating at a 0t of less than or equal to 1. Hybridization of the Hae III dimer to gels eluted on to filters demonstrated that the multiple Hae III fragments and Eco R1 fragments contained compatible sequences. These sequences may comprise a distinct subclass of DNA.

MeSH Terms
Adult Base Sequence Brain Chemistry Brain Neoplasms/analysis DNA DNA Restriction Enzymes DNA, Satellite Embryo, Mammalian Escherichia coli/enzymology Female Haemophilus influenzae/enzymology Humans Male Molecular Weight Nucleic Acid Hybridization Organ Specificity Placenta/analysis Pregnancy
Chemicals
DNA, Satellite DNA DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Manuelidis L
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22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.11.3063
Published
1976-11-00
Pages
3063-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343151
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.11.3063 PMC Full Text View journal details
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