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PMID: 6246282 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Modification of avian sarcoma proviral DNA sequences in nonpermissive XC cells but not in permissive chicken cells.

Journal of virology ·Vol. 34 ·No. 2 ·1980-05-00 ·Pages 569-72

Guntaka RV, Rao PY, Mitsialis SA, Katz R

Abstract

For the first time, we present evidence with restriction enzymes HpaII and MspI which indicates that the proviral DNA sequence of avian sarcoma virus is modified by methylation in a nonpermissive rat cell line but not in permissive chicken cells. Some of the endogenous viral sequences in the permissive cells were also methylated. No 5-methylcytosine could be detected in the unintegrated viral DNA.

MeSH Terms
5-Methylcytosine Alpharetrovirus/analysis,genetics,physiology Animals Base Sequence Cell Line Cell Transformation, Viral Chick Embryo Cytosine/analogs & derivatives,analysis DNA Restriction Enzymes/pharmacology DNA, Viral/analysis,genetics Methylation Rats Recombination, Genetic
Chemicals
DNA, Viral 5-Methylcytosine Cytosine DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guntaka R V
Rao P Y
Mitsialis S A
Katz R
References (20)
20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.34.2.569-572.1980
Published
1980-05-00
Pages
569-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288737
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.34.2.569-572.1980 PMC Full Text View journal details
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