Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 11 ·1973-11-00 ·Pages 3067-71

Varmus HE, Vogt PK, Bishop JM

Abstract

A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated.

MeSH Terms

Animals Avian Sarcoma Viruses/metabolism Cell Line Cell Transformation, Neoplastic Centrifugation, Density Gradient DNA/analysis DNA Nucleotidyltransferases DNA, Viral/biosynthesis,isolation & purification Ducks Embryo, Mammalian Embryo, Nonmammalian Fibroblasts Kinetics Mice Mice, Inbred BALB C Nucleic Acid Denaturation Nucleic Acid Renaturation Tritium

Chemicals

DNA, Viral Tritium DNA DNA Nucleotidyltransferases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Varmus H E

Vogt P K

Bishop J M

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.11.3067

Published

1973-11-00

Pages

3067-71

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427171

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.11.3067 PMC Full Text View journal details

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Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts.

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