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PMID: 6190135 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sequence GGCmCGG is resistant to MspI cleavage.

Nucleic acids research ·Vol. 11 ·No. 11 ·1983-06-11 ·Pages 3559-69

Busslinger M, deBoer E, Wright S, Grosveld FG, Flavell RA

Abstract

MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.

MeSH Terms

Animals Base Sequence Cloning, Molecular DNA DNA Restriction Enzymes/metabolism Deoxyribonuclease HpaII Genes Globins/genetics Humans L Cells/enzymology Methylation Mice Nucleic Acid Hybridization Substrate Specificity Thymidine Kinase/deficiency

Chemicals

Globins DNA Thymidine Kinase DNA Restriction Enzymes Deoxyribonuclease HpaII

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Busslinger M

deBoer E

Wright S

Grosveld F G

Flavell R A

References (14)

14 references, click to expand

Specificity of Hpa II and Hae III DNA methylases.
Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.
J Mol Biol. 1978 Jan 5;118(1):27-47 PMID: 625056
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
Nucleic Acids Res. 1978 Sep;5(9):3231-6 PMID: 704354
DNA methylation in the human gamma delta beta-globin locus in erythroid and nonerythroid tissues.
Cell. 1980 Apr;19(4):947-58 PMID: 6247075
The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
Cell. 1980 May;20(1):119-30 PMID: 6446404
Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
Cell. 1980 Oct;21(3):627-38 PMID: 7438203
Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.
Gene. 1980 Oct;11(1-2):129-48 PMID: 6254849
A novel type of secondary modification of two CCGG residues in the human gamma delta beta-globin gene locus.
Nucleic Acids Res. 1980 Oct 24;8(20):4563-74 PMID: 6255424
The 3' untranslated regions of the duplicated human alpha-globin genes are unexpectedly divergent.
Cell. 1980 Nov;22(2 Pt 2):371-7 PMID: 7448866
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23 PMID: 6262820
Clonal inheritance of the pattern of DNA methylation in mouse cells.
Proc Natl Acad Sci U S A. 1982 Jan;79(1):61-5 PMID: 6459581
DNA methylation in chicken alpha-globin gene expression.
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5332-6 PMID: 6291038
The construction of cosmid libraries which can be used to transform eukaryotic cells.
Nucleic Acids Res. 1982 Nov 11;10(21):6715-32 PMID: 6294612
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
Gene. 1982 Oct;19(3):269-76 PMID: 6295880

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/11.11.3559

Published

1983-06-11

Pages

3559-69

Language

English

Region

England

NLM ID

0411011

PMCID

PMC325986

Subset

External Links

PubMed source DOI: 10.1093/nar/11.11.3559 PMC Full Text View journal details

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The sequence GGCmCGG is resistant to MspI cleavage.

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