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PMID: 3041022 Published · ppublish English Journal Article

Nucleotide sequence of a complete mouse intracisternal A-particle genome: relationship to known aspects of particle assembly and function.

Journal of virology ·Vol. 61 ·No. 10 ·1987-10-00 ·Pages 3020-9

Mietz JA, Grossman Z, Lueders KK, Kuff EL

Abstract

The 7,095-nucleotide sequence of a mouse genomic intracisternal A-particle (IAP) element, MIA14, is reported. MIA14 is known to be colinear with IAP 35S RNA and to contain functional long terminal repeats. Its internal genetic organization was determined by comparisons with a homologous Syrian hamster element and the related retroviruses simian retrovirus 1 (simian type D) and Rous sarcoma virus (avian type C). MIA14 contains a gag-protease open reading frame of 827 codons and a pol region of 867 codons entered by a frame shift of -1. The env region of 1,100 base pairs has multiple stop codons in all reading frames, consistent with the failure thus far to detect IAP-related glycosylated envelope components. RNA transcribed in vitro from a cDNA clone containing a closely homologous gag-protease open reading frame was translated in a cell-free system. The main product was a 73-kilodalton polypeptide immunoprecipitable with antiserum against the authentic IAP gag-related structural protein p73. Rather than ending at the gag-protease boundary, p73 appears to contain 7 to 8 kilodaltons of peptide encoded by the protease domain, a peculiarity possibly related to the observed impairment of normal protein processing in IAPs. The N-terminal 217 codons of gag are unique to murine IAPs and may have been contributed by recombination with a cellular gene. The mouse-specific region of gag encodes a hydrophobic signal peptide with an atypical cleavage site. Delayed cleavage of this peptide could result in anchoring of newly synthesized p73 to the endoplasmic reticulum membrane and restriction of particle assembly to this site.

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses/genetics Base Sequence Cloning, Molecular Codon/analysis Cricetinae DNA, Viral/analysis Defective Viruses/genetics Genes, Intracisternal A-Particle Genes, Viral Mesocricetus Mice Mice, Inbred BALB C Protein Biosynthesis Proto-Oncogenes RNA, Viral/biosynthesis,genetics Retroviridae/genetics Sequence Homology, Nucleic Acid Software
Chemicals
Codon DNA, Viral RNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mietz J A
Grossman Z
Lueders K K
Kuff E L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.10.3020-3029.1987
Published
1987-10-00
Pages
3020-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255875
Subset
IM
Databases
GENBANK
K01572, K01573, M17551
External Links
PubMed source DOI: 10.1128/JVI.61.10.3020-3029.1987 PMC Full Text View journal details
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