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PMID: 2884637 Published · ppublish English Comparative Study Journal Article

Nearly identical members of the heterogeneous IAP gene family are expressed in thymus of different mouse strains.

Nucleic acids research ·Vol. 15 ·No. 9 ·1987-05-11 ·Pages 3823-34

Grossman Z, Mietz JA, Kuff EL

Abstract

Poly(A)RNAs prepared from the thymuses of C57BL/6J and DBA/2J mice were used to construct cDNA libraries in the bacterial expression vector lambda gt11. The libraries were scanned first for protein production with polyvalent antiserum prepared against the 73kDa gag protein of mouse intracisternal A-particles (IAP). Reactive plaques were crossed-screened by hybridization with an IAP-specific DNA probe. Two IAP-specific protein-producing plaques were obtained from the C57BL/6 library and 4 from the DBA/2 library. One C57BL/6 cDNA clone (B12) and two DBA cDNA clones (D8 and D20) were sequenced in their entirety. Clones B12 and D8 were remarkably similar, particularly when compared to the 6 other IAP elements that have been sequenced thus far. We discuss the evidence which leads us to suggest that these clones may be derived from allelic IAP elements expressed in mouse thymus.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence DNA/genetics Gene Expression Regulation Gene Products, gag Genes, Intracisternal A-Particle Mice Mice, Inbred C57BL/genetics Mice, Inbred DBA/genetics Poly A/genetics,isolation & purification Proto-Oncogenes RNA, Messenger/genetics,isolation & purification Retroviridae Proteins/biosynthesis,genetics Sequence Homology, Nucleic Acid Thymus Gland/analysis
Chemicals
Gene Products, gag RNA, Messenger Retroviridae Proteins Poly A DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grossman Z
Mietz J A
Kuff E L
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.9.3823
Published
1987-05-11
Pages
3823-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340784
Subset
IM
Databases
GENBANK
X05545, X05546, Y00153
External Links
PubMed source DOI: 10.1093/nar/15.9.3823 PMC Full Text View journal details
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