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PMID: 3016667 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the intracisternal A-particle genome inserted 5' to the interleukin-3 gene of the leukemia cell line WEHI-3B.

Nucleic acids research ·Vol. 14 ·No. 14 ·1986-07-25 ·Pages 5901-18

Ymer S, Tucker WQ, Campbell HD, Young IG

Abstract

The nucleotide sequence of the intracisternal A-particle genome IAP-IL3 is presented. This IAP element was found to have inserted upstream of the promoter of the interleukin-3 gene of the leukemia cell line WEHI-3B. IAP-IL3 is 5095 bp in length, with identical long terminal repeats (LTRs) of 337 bp. The LTRs show many of the conserved sequence elements identified in other retroviruses. Comparison with other available sequences of IAP genomes indicates that IAP-IL3 is a deleted type I element. It carries a deletion covering the 3' end of the putative IAP gag gene and extending into the 5' end of the putative IAP pol gene. IAP-IL3 has extensive sequence homology with an IgE-binding factor cDNA and evidence is presented indicating that it was derived from a member of the mouse IAP sequence family. Comparison between the pol region of IAP-IL3 and other retroviruses suggests that IAP-IL3 is most closely related to type B and type D retroviruses.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Genes Genes, Intracisternal A-Particle Interleukin-3 Leukemia, Experimental Lymphokines/genetics Mice Proto-Oncogenes
Chemicals
DNA Transposable Elements Interleukin-3 Lymphokines DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ymer S
Tucker W Q
Campbell H D
Young I G
References (54)
54 references, click to expand
  1. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  2. Intracisternal A-particle genes in Mus musculus: a conserved family of retrovirus-like elements.
    Mol Cell Biol. 1981 Mar;1(3):216-27 PMID: 6821514
  3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  4. Studies on the relationship between deoxyribonucleic acid polymerase activity and intracisternal A-type particles in mouse myeloma.
    Biochemistry. 1974 Mar 12;13(6):1087-94 PMID: 4360775
  5. Synthesis and turnover of intracisternal A-particle structural protein in cultured neuroblastoma cells.
    J Biol Chem. 1975 Jul 10;250(13):5192-9 PMID: 168201
  6. Sequences associated with intracisternal A particles are reiterated in the mouse genome.
    Cell. 1977 Dec;12(4):963-72 PMID: 597866
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. The primary structure of rabbit, calf and bovine liver tRNAPhe.
    Biochim Biophys Acta. 1978 Jan 26;517(1):133-49 PMID: 414781
  9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  10. RNA associated with murine intracisternal type A particles codes for the main particle protein.
    J Virol. 1978 Jul;27(1):118-26 PMID: 691107
  11. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
    Anal Biochem. 1979 Oct 1;98(2):305-9 PMID: 386835
  12. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  13. Regulatory role of IgE-binding factors from rat T lymphocytes. I. Mechanism of enhancement of IgE response by IgE-potentiating factor.
    J Immunol. 1980 Jul;125(1):148-54 PMID: 6966648
  14. Sequence organization of cloned intracisternal A particle genes.
    Cell. 1980 Sep;21(2):465-73 PMID: 6250723
  15. Regulatory role of IgE-binding factors from rat T lymphocytes. III. IgE-specific suppressive factor with IgE-binding activity.
    J Immunol. 1980 Oct;125(4):1442-8 PMID: 6967903
  16. Intracisternal A-particle genes: identification in the genome of Mus musculus and comparison of multiple isolates from a mouse gene library.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3571-5 PMID: 6251469
  17. A new computer method for the storage and manipulation of DNA gel reading data.
    Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
  18. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  19. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  20. Sequences homologous to retrovirus-like genes of the mouse are present in multiple copies in the Syrian hamster genome.
    Nucleic Acids Res. 1981 Nov 25;9(22):5917-30 PMID: 7198224
  21. Structure, variation and synthesis of retrovirus long terminal repeat.
    Cell. 1981 Nov;27(1 Pt 2):1-3 PMID: 7326747
  22. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  23. Form and function of retroviral proviruses.
    Science. 1982 May 21;216(4548):812-20 PMID: 6177038
  24. Formation of rat IgE-binding factors by rat-mouse T cell hybridomas.
    J Immunol. 1982 Aug;129(2):509-14 PMID: 6979575
  25. Differing populations of intracisternal A-particle genes in myeloma tumors and mouse subspecies.
    J Virol. 1982 May;42(2):411-21 PMID: 6283165
  26. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  27. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  28. Multiple point mutations affecting the simian virus 40 enhancer.
    Science. 1983 Feb 11;219(4585):626-31 PMID: 6297005
  29. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  30. Intracisternal A-particle genes as movable elements in the mouse genome.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992-6 PMID: 6300886
  31. Nucleotide sequence of bacteriophage lambda DNA.
    J Mol Biol. 1982 Dec 25;162(4):729-73 PMID: 6221115
  32. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.
    Anal Biochem. 1983 Feb 15;129(1):216-23 PMID: 6305233
  33. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  34. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  35. Activation of the c-mos oncogene in a mouse plasmacytoma by insertion of an endogenous intracisternal A-particle genome.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7118-22 PMID: 6316344
  36. A computer program to enter DNA gel reading data into a computer.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):499-503 PMID: 6546430
  37. Nucleotide sequences of the retroviral long terminal repeats and their adjacent regions.
    Nucleic Acids Res. 1984 Feb 24;12(4):1767-78 PMID: 6322120
  38. Contacts between hormone receptor and DNA double helix within a glucocorticoid regulatory element of mouse mammary tumor virus.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3029-33 PMID: 6328488
  39. Transcription of intracisternal A-particle genes in mouse myeloma and Ltk- cells.
    J Virol. 1984 Oct;52(1):29-36 PMID: 6481855
  40. A retroviral provirus closely associated with the Ren-2 gene of DBA/2 mice.
    Nucleic Acids Res. 1984 Nov 26;12(22):8579-93 PMID: 6095203
  41. Mechanism of activation of the mouse c-mos oncogene by the LTR of an intracisternal A-particle gene.
    EMBO J. 1984 Dec 1;3(12):2937-41 PMID: 6098457
  42. Transposition of two different intracisternal A particle elements into an immunoglobulin kappa-chain gene.
    Mol Cell Biol. 1984 Dec;4(12):2565-72 PMID: 6098810
  43. Intracisternal A-particle gene expression in normal mouse thymus tissue: gene products and strain-related variability.
    Mol Cell Biol. 1985 Mar;5(3):474-83 PMID: 2859519
  44. cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2460-4 PMID: 3873067
  45. Nucleotide sequences of murine intracisternal A-particle gene LTRs have extensive variability within the R region.
    Nucleic Acids Res. 1985 Jan 11;13(1):289-302 PMID: 2987792
  46. A new class of endogenous human retroviral genomes.
    Science. 1985 Jun 7;228(4704):1208-11 PMID: 2408338
  47. Transposable elements in eukaryotes.
    Int Rev Cytol. 1985;93:281-326 PMID: 2989205
  48. Cloning and nucleotide sequence of the murine interleukin-3 gene.
    Eur J Biochem. 1985 Jul 15;150(2):297-304 PMID: 3926497
  49. Nucleotide sequence of the Syrian hamster intracisternal A-particle gene: close evolutionary relationship of type A particle gene to types B and D oncovirus genes.
    J Virol. 1985 Aug;55(2):387-94 PMID: 2991563
  50. Constitutive synthesis of interleukin-3 by leukaemia cell line WEHI-3B is due to retroviral insertion near the gene.
    Nature. 1985 Sep 19-25;317(6034):255-8 PMID: 2413359
  51. Regulation of IgE synthesis.
    Annu Rev Immunol. 1984;2:159-82 PMID: 6085750
  52. Retroviral gag and DNA endonuclease coding sequences in IgE-binding factor gene.
    Nature. 1985 Nov 28-Dec 4;318(6044):388-9 PMID: 3877875
  53. Structural analysis of type II variants within the mouse intracisternal A-particle sequence family.
    Nucleic Acids Res. 1986 Feb 11;14(3):1495-510 PMID: 2419836
  54. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):218-22 PMID: 4333040
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.14.5901
Published
1986-07-25
Pages
5901-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311599
Subset
IM
Databases
GENBANK
X04120
External Links
PubMed source DOI: 10.1093/nar/14.14.5901 PMC Full Text View journal details
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