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PMID: 3873067 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2460-4

Martens CL, Huff TF, Jardieu P, Trounstine ML, Coffman RL, Ishizaka K, Moore KW

Abstract

cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of approximately equal to 60 and approximately equal to 11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Glycoproteins/genetics Hybridomas/immunology Lymphokines/genetics Mice Prostatic Secretory Proteins Rats T-Lymphocytes/immunology
Chemicals
Glycoproteins Lymphokines Prostatic Secretory Proteins beta-microseminoprotein immunoglobulin-binding factors DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Martens C L
Huff T F
Jardieu P
Trounstine M L
Coffman R L
Ishizaka K
Moore K W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.8.2460
Published
1985-04-00
Pages
2460-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397578
Subset
IM
Grants
NIAID NIH HHS · AI-14784 · United States
NIAID NIH HHS · T-32 AI-07056 · United States
Databases
GENBANK
M10062
External Links
PubMed source DOI: 10.1073/pnas.82.8.2460 PMC Full Text View journal details
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