Home LiteratureArticle Details
PMID: 6326111 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cell-type-specific cDNA probes and the murine I region: the localization and orientation of Ad alpha.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 7 ·1984-04-00 ·Pages 2194-8

Davis MM, Cohen DI, Nielsen EA, Steinmetz M, Paul WE, Hood L

Abstract

Labeled cDNA probes highly enriched for B-cell-specific (B-T) and T-suppressor-cell-specific (Ts-B) sequences were used to screen a set of genomic cosmid clones spanning 230 kilobases of the murine immune response (I) region. With the B-cell derived probe, four I-region genes were detectable (A beta, E beta, E beta 2, and E alpha), as well as an additional (fifth) region of hybridization. The T-cell probe, prepared from a putative I-J positive suppressor cell hybridoma, was negative in a parallel experiment. A genomic fragment corresponding to the new region of hybridization seen with the B-cell cDNA probe identified a discrete mRNA species in RNA blotting analysis that had a pattern of expression strikingly similar to E alpha mRNA in a variety of lymphoid tumor lines. This fragment was also used to isolate cDNA clones from a library highly enriched (20-40 times) for B-cell-specific sequences (selected cDNA cloning). DNA sequence analysis of one of these cDNA clones indicates that this gene encodes the A alpha molecule of the d haplotype (Ad alpha). These findings establish that the order of the class II genes in the Ia complex is as follows: centromere-A beta-A alpha-E beta-E beta 2-E alpha.

MeSH Terms
Animals B-Lymphocytes/immunology Base Sequence Cell Line Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Genes Genes, MHC Class II Major Histocompatibility Complex Mice Nucleic Acid Hybridization Plasmids T-Lymphocytes, Regulatory/immunology
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Davis M M
Cohen D I
Nielsen E A
Steinmetz M
Paul W E
Hood L
References (35)
35 references, click to expand
  1. Isolation of developmentally regulated genes from Dictyostelium.
    Dev Biol. 1980 Oct;79(2):409-18 PMID: 7429011
  2. 5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains.
    Cell. 1980 Nov;22(1 Pt 1):187-96 PMID: 6775817
  3. Expression of J chain RNA in cell lines representing different stages of B lymphocyte differentiation.
    Cell. 1981 Feb;23(2):369-78 PMID: 6781757
  4. Mouse immunoglobulin D: construction and characterization of a cloned delta chain cDNA.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7405-9 PMID: 6784124
  5. Ir gene function in an I-A subregion mutant B6.C-H-2bm12.
    J Exp Med. 1981 Feb 1;153(2):464-9 PMID: 6787167
  6. A variant alpha-chain in an Ia molecule from the I-A subregion of the mouse major histocompatibility complex: a possible intragenic recombination.
    J Immunol. 1981 Oct;127(4):1472-7 PMID: 6792275
  7. Selective loss of antigen-specific Ir gene function in IA mutant B6.C-H-2bm12 is an antigen presenting cell defect.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6406-10 PMID: 7031650
  8. Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.
    Nucleic Acids Res. 1982 Mar 11;10(5):1799-810 PMID: 7071022
  9. Identification of a fourth class of proteins linked to the murine major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1982 May;79(9):3001-5 PMID: 6953443
  10. A molecular map of the immune response region from the major histocompatibility complex of the mouse.
    Nature. 1982 Nov 4;300(5887):35-42 PMID: 6290895
  11. Regions of allelic hypervariability in the murine A alpha immune response gene.
    Cell. 1983 Aug;34(1):169-77 PMID: 6309407
  12. RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5704-8 PMID: 6193520
  13. Extensive polymorphism surrounding the murine Ia A beta chain gene.
    J Immunol. 1983 Oct;131(4):2025-31 PMID: 6311906
  14. Differential gene expression in the gastrula of Xenopus laevis.
    Science. 1983 Oct 14;222(4620):135-9 PMID: 6688681
  15. Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.
    Nature. 1984 Mar 8-14;308(5955):149-53 PMID: 6199676
  16. Genes of the major histocompatibility complex of the mouse.
    Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
  17. Genetic control of specific immune responses.
    Adv Immunol. 1969;11:31-74 PMID: 4908392
  18. Inhibition of immune responses in vitro by specific antiserums to Ia antigens.
    Science. 1975 Apr 18;188(4185):268-70 PMID: 1118728
  19. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  20. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
    Nucleic Acids Res. 1976 Jan;3(1):101-16 PMID: 765970
  21. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera.
    J Immunol. 1976 Aug;117(2):531-40 PMID: 1084902
  22. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes.
    J Exp Med. 1976 Sep 1;144(3):699-712 PMID: 1085338
  23. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex.
    J Exp Med. 1976 Sep 1;144(3):713-25 PMID: 1085339
  24. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  25. Immunochemical evidence for three Ia loci in the I-region of the H-2 complex.
    J Exp Med. 1977 Dec 1;146(6):1549-60 PMID: 72779
  26. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  27. Characteristics of BALB/C T cell lymphomas grown as continuous in vitro lines.
    J Immunol. 1978 Jul;121(1):339-44 PMID: 307579
  28. Two-gene control of the expression of a murine Ia antigen.
    J Exp Med. 1978 Oct 1;148(4):925-39 PMID: 100572
  29. Establishment and characterization of BALB/c lymphoma lines with B cell properties.
    J Immunol. 1979 Feb;122(2):549-54 PMID: 310843
  30. Organization of the immune response genes.
    Science. 1979 Oct 19;206(4416):292-7 PMID: 113876
  31. Antigenic marker on a functional subpopulation of B cells, controlled by the I-A subregion of the H-2 complex.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3460-3 PMID: 91165
  32. Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma.
    Nature. 1980 Jan 10;283(5743):227-8 PMID: 6444248
  33. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  34. Partial N-terminal amino acid sequence analyses and comparative tryptic peptide maps of murine Ia molecules encoded by the I-A subregion.
    Eur J Immunol. 1979 Dec;9(12):955-63 PMID: 121573
  35. Genomic organization of rat prolactin and growth hormone genes.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4583-7 PMID: 6254031
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.7.2194
Published
1984-04-00
Pages
2194-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345464
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.7.2194 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2