Home LiteratureArticle Details
PMID: 6166005 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 4 ·1981-04-00 ·Pages 2253-7

Parnes JR, Velan B, Felsenfeld A, Ramanathan L, Ferrini U, Appella E, Seidman JG

Abstract

We have isolated three cDNA clones for beta 2-microglobulin, the small subunit of the major histocompatibility antigens. beta 2-Microglobulin makes up less than 0.1% of mouse liver protein, and its mRNA is approximately 0.03% of liver poly(A)+ mRNA. The cDNA clones were identified by screening 1400 cDNA clones made from 9--10S mouse liver poly(A)+ mRNA. The procedure for screening the cDNA clones involved binding pooled plasmid DNA to nitrocellulose filters and testing the ability of each filter to select beta 2-microglobulin mRNA. The filter-selected mRNAs were assayed for their ability to direct the synthesis of beta 2-microglobulin in translation reactions in vitro. The isolated clones were shown by nucleotide sequence analysis to encode beta 2-microglobulin. The positive-selection--hybridization assay has been modified to facilitate the screening of large numbers of cDNA clones, and the modified assay should allow the isolation of cDNAs corresponding to any mRNA whose in vitro translation products can be immunoprecipitated. These modifications are of particular value in the isolation of cDNA clones corresponding to rare species of mRNA.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Beta-Globulins/genetics Cloning, Molecular/methods DNA, Recombinant Genes Liver/analysis Mice Plasmids RNA, Messenger/genetics beta 2-Microglobulin/genetics
Chemicals
Beta-Globulins DNA, Recombinant RNA, Messenger beta 2-Microglobulin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Parnes J R
Velan B
Felsenfeld A
Ramanathan L
Ferrini U
Appella E
Seidman J G
References (33)
33 references, click to expand
  1. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  2. The protein products of the murine 17th chromosome: genetics and structure.
    Adv Immunol. 1978;26:147-93 PMID: 99995
  3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  4. Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain.
    Nucleic Acids Res. 1979 Jul 25;6(10):3305-21 PMID: 113776
  5. Cell-free synthesis and membrane insertion of mouse H-2Dd histocompatibility antigen and beta 2-microglobulin.
    Cell. 1979 Aug;17(4):759-69 PMID: 90552
  6. Cell-free synthesis and segregation of beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3651-5 PMID: 91168
  7. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
  8. The evolution and sequence comparison of two recently diverged mouse chromosomal beta--globin genes.
    Cell. 1979 Nov;18(3):865-73 PMID: 519759
  9. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
    Nature. 1980 Mar 27;284(5754):316-20 PMID: 6987533
  10. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  11. Molecular cloning of mouse immunoglobulin heavy chain messenger ribonucleic acids coding for mu, alpha, gamma 1, gamma 2a, and gamma 3 chains.
    Biochemistry. 1980 Jun 10;19(12):2711-9 PMID: 6249338
  12. Genetic polymorphism of murine beta 2-microglobulin detected biochemically.
    Immunogenetics. 1980 Jul;11(1):93-5 PMID: 6160099
  13. Nucleotide sequence of a cloned structural gene coding for a precursor of pancreatic somatostatin.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5869-73 PMID: 6108560
  14. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  15. Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):554-8 PMID: 6165997
  16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.
    Nature. 1976 Mar 18;260(5548):221-5 PMID: 768776
  19. The component fragments obtained by acid dissociation of papain-solubilized H-2 molecules.
    J Immunogenet. 1976 Feb;3(1):35-47 PMID: 932465
  20. A mouse plasma substance carrying beta2-microglobulin activity and lacking in H-2 alloantigenic activity.
    J Immunogenet. 1976 Apr;3(2):123-34 PMID: 58948
  21. Partial amino acid sequence of mouse beta2-microglobulin.
    Biochem Biophys Res Commun. 1976 May 17;70(2):425-30 PMID: 59595
  22. Amplification and characterization of a beta-globin gene synthesized in vitro.
    Cell. 1976 Jun;8(2):163-82 PMID: 61066
  23. Absence of a serologically detectable association of murine beta2-microglobulin with the embryonic F9 antigen.
    Transplantation. 1976 Nov;22(5):467-73 PMID: 63168
  24. The ovalbumin gene. Insertion of ovalbumin gene sequences in chimeric bacterial plasmids.
    J Biol Chem. 1977 Mar 25;252(6):1840-3 PMID: 321443
  25. Rat insulin genes: construction of plasmids containing the coding sequences.
    Science. 1977 Jun 17;196(4296):1313-9 PMID: 325648
  26. Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.
    Nucleic Acids Res. 1977 Jul;4(7):2389-406 PMID: 333389
  27. Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.
    Gene. 1977 May;1(3-4):229-39 PMID: 338418
  28. Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid.
    Nature. 1978 Feb 9;271(5645):582-5 PMID: 414143
  29. A sensitive radioimmunoassay for detecting products translated from cloned DNA fragments.
    Cell. 1978 Apr;13(4):681-9 PMID: 418881
  30. Immunological screening method to detect specific translation products.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2746-9 PMID: 96441
  31. Construction and identification by positive hybridization-translation of a bacterial plasmid containing a rat growth hormone structural gene sequence.
    Nucleic Acids Res. 1978 Jun;5(6):2039-53 PMID: 353736
  32. A bacterial clone synthesizing proinsulin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3727-31 PMID: 358198
  33. Immunoglobulin heavy chain gene organization in mice: analysis of a myeloma genomic clone containing variable and alpha constant regions.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):857-61 PMID: 106394
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.4.2253
Published
1981-04-00
Pages
2253-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319323
Subset
IM
Grants
NIAID NIH HHS · 1F32 AI05932-01 · United States
Databases
GENBANK
M10389, M10416
External Links
PubMed source DOI: 10.1073/pnas.78.4.2253 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2