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PMID: 325648 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rat insulin genes: construction of plasmids containing the coding sequences.

Science (New York, N.Y.) ·Vol. 196 ·No. 4296 ·1977-06-17 ·Pages 1313-9

Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM

Abstract

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.

MeSH Terms

Animals Base Sequence Codon DNA/isolation & purification DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis,metabolism DNA, Recombinant/analysis,metabolism Escherichia coli Extrachromosomal Inheritance Genes Islets of Langerhans/analysis Plasmids Proinsulin/biosynthesis Protein Precursors/biosynthesis RNA, Messenger/isolation & purification Rats Transformation, Genetic

Chemicals

Codon DNA, Bacterial DNA, Recombinant Protein Precursors RNA, Messenger DNA Proinsulin DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Ullrich A

Shine J

Chirgwin J

Pictet R

Tischer E

Rutter W J

Goodman H M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.325648

Published

1977-06-17

Pages

1313-9

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

J00747

External Links

PubMed source DOI: 10.1126/science.325648 View journal details

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Rat insulin genes: construction of plasmids containing the coding sequences.

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