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PMID: 113776 Published · ppublish English Journal Article

Sequence analysis of cloned cDNA encoding part of an immunoglobulin heavy chain.

Nucleic acids research ·Vol. 6 ·No. 10 ·1979-07-25 ·Pages 3305-21

Rogers J, Clarke P, Salser W

Abstract

The recombinant plasmid pH21-1 consists of mouse-derived complementary DNA (cDNA) in the E. coli plasmid pMB9. The mouse insertion has been completely sequenced, and encodes the CH3 domain and half the CH2 domain of the immunoglobulin gamma1 heavy chain. The predicted amino acid sequence differs at several positions from that previously published for this protein. The pattern of codon usage resembles that in some other eukaryotic messenger RNAs. A computer program has been used to predict the optimum secondary structure for the mRNA encoding the CH3 domain and the inter-domain junction.

MeSH Terms
Animals Base Sequence Containment of Biohazards DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/metabolism Genetic Code Immunoglobulin Heavy Chains/biosynthesis Mice Plasmids Protein Biosynthesis Transcription, Genetic
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rogers J
Clarke P
Salser W
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.10.3305
Published
1979-07-25
Pages
3305-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327935
Subset
IM
Databases
GENBANK
J00453, V00795
External Links
PubMed source DOI: 10.1093/nar/6.10.3305 PMC Full Text View journal details
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