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PMID: 802513 Published · ppublish English Journal Article

Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.

Nucleic acids research ·Vol. 2 ·No. 12 ·1975-12-00 ·Pages 2365-78

Rougeon F, Kourilsky P, Mach B

Abstract

Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired and dC tails added, to permit reconstitution of the EcoRI sites upon annealing with the dG elongated globin DNA. Transformation of E. coli with the globin-plasmid DNA hybrid has yielded a clone which harbours a recombinant plasmid (pCR1-betaG1), as demonstrated by hybridization experiments with radioactive globin cDNA. The sequence carried by the recombinant plasmid corresponds to part of the gene sequence coding for the beta chain of rabbit globin. Circular DNA of the purified recombinant plasmid exhibits sensitivity to EcoRI.

MeSH Terms
Animals DNA Restriction Enzymes DNA, Circular DNA, Recombinant Escherichia coli/genetics Globins/biosynthesis,genetics Nucleic Acid Hybridization Plasmids Rabbits Transformation, Genetic
Chemicals
DNA, Circular DNA, Recombinant Globins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rougeon F
Kourilsky P
Mach B
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.12.2365
Published
1975-12-00
Pages
2365-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343603
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.12.2365 PMC Full Text View journal details
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