DNA nucleotide sequence restricted by the RI endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3448-52

Hedgpeth J, Goodman HM, Boyer HW

Abstract

The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed from a pancreatic DNase digest. The following sequence of nucleotides adjacent to the RI break made in lambda DNA was deduced from these data and from the 3'-dinucleotide sequence and nearest-neighbor analysis obtained from repair synthesis with the DNA polymerase of Rous sarcoma virus [Formula: see text] The RI endonuclease cleavage of the phosphodiester bonds (indicated by arrows) generates 5'-phosphoryls and short cohesive termini of four nucleotides, (p)A(p)A(p)T(p)T. The most striking feature of the sequence is its symmetry.

MeSH Terms

Adenine Nucleotides/analysis Alkaline Phosphatase/metabolism Avian Sarcoma Viruses/enzymology Base Sequence Coliphages Cytosine Nucleotides/analysis DNA Nucleotidyltransferases/metabolism DNA, Viral/biosynthesis,metabolism Deoxyribonucleases/metabolism Drug Resistance, Microbial Endonucleases/metabolism Escherichia coli/enzymology Extrachromosomal Inheritance Nucleotides/analysis,metabolism Oligonucleotides/analysis Phosphoric Diester Hydrolases/metabolism Phosphorus Isotopes Polynucleotides/analysis Templates, Genetic Thymine Nucleotides/analysis

Chemicals

Adenine Nucleotides Cytosine Nucleotides DNA, Viral Nucleotides Oligonucleotides Phosphorus Isotopes Polynucleotides Thymine Nucleotides DNA Nucleotidyltransferases Deoxyribonucleases Endonucleases Alkaline Phosphatase Phosphoric Diester Hydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hedgpeth J

Goodman H M

Boyer H W

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.11.3448

Published

1972-11-00

Pages

3448-52

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389791

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.11.3448 PMC Full Text View journal details

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DNA nucleotide sequence restricted by the RI endonuclease.

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