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PMID: 100767 Published · ppublish English Journal Article

Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.

Nucleic acids research ·Vol. 5 ·No. 9 ·1978-09-00 ·Pages 3113-28

Wall R, Gilmore-Hebert M, Higuchi R, Komaromy M, Paddock G, Strommer J, Salser W

Abstract

Recombinant DNA clones have been generated from mouse myeloma MOPC 21 immunoglobulin kappa light chain mRNA. Complementary DNA (cDNA) synthesized on kappa light chain mRNA by reverse transcriptase was made double stranded and inserted into the bacterial plasmid vector, pMB9. Approximately 70 tetracycline-resistant transformed colonies containing kappa light chain mRNA sequences were identified by colony hybridization. Five of these recombinant clones were selected and characterized. Three clones contain both kappa light chain constant and variable region sequences. Two of these three recombinant clones have been shown to include all of the kappa light chain constant and variable region coding sequences. Another of the five selected recombinant clones contain kappa light chain constant region sequences. The remaining characterized clone appears to be derived from sequences at the 5'-end of kappa light chain mRNA, possibly extending to the terminal cap structure.

MeSH Terms
Base Sequence Cell Line DNA Restriction Enzymes/metabolism DNA, Recombinant Immunoglobulin Constant Regions/genetics Immunoglobulin Light Chains/genetics Immunoglobulin Variable Region/genetics Immunoglobulin kappa-Chains/genetics Myeloma Proteins/genetics Plasmids RNA, Messenger/genetics
Chemicals
DNA, Recombinant Immunoglobulin Constant Regions Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin kappa-Chains Myeloma Proteins RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wall R
Gilmore-Hebert M
Higuchi R
Komaromy M
Paddock G
Strommer J
Salser W
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.9.3113
Published
1978-09-00
Pages
3113-28
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342235
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.9.3113 PMC Full Text View journal details
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