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PMID: 4139714 Published · ppublish English Journal Article

Nucleotide sequence analysis of RNA synthesized from rabbit globin complementary DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3502-6

Poon R, Paddock GV, Heindell H, Whitcome P, Salser W, Kacian D, Bank A, Gambino R, Ramirez F

Abstract

Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of (32)P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T(1) and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the alpha or beta globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.

MeSH Terms
Alkaline Phosphatase Amino Acid Sequence Animals Base Sequence DNA Globins Phosphoric Diester Hydrolases Phosphorus Radioisotopes RNA/analysis,biosynthesis RNA, Messenger/analysis RNA-Directed DNA Polymerase Rabbits Ribonucleases Templates, Genetic
Chemicals
Phosphorus Radioisotopes RNA, Messenger RNA Globins DNA RNA-Directed DNA Polymerase Ribonucleases Alkaline Phosphatase Phosphoric Diester Hydrolases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Poon R
Paddock G V
Heindell H
Whitcome P
Salser W
Kacian D
Bank A
Gambino R
Ramirez F
References (35)
35 references, click to expand
  1. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  2. Topography of nucleic acid helices in solutions. IV. Effect of polyamines on RNase-catalyzed hydrolysis of polyadenylic acid.
    Biopolymers. 1968;6(2):255-68 PMID: 5641421
  3. Isolation of an RNA with the properties of haemoglobin messenger.
    Nature. 1969 Mar 29;221(5187):1217-22 PMID: 5773836
  4. Separation and characterization of the subunits of ribonucleic acid polymerase.
    J Biol Chem. 1969 Nov 25;244(22):6168-76 PMID: 4900511
  5. RNA-dependent DNA polymerase in virions of RNA tumour viruses.
    Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300
  6. RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
    Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
  7. Synthesis of DNA complements of natural RNAs: a general approach.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2843-5 PMID: 4330945
  8. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):264-8 PMID: 4333043
  9. Properties of isoaccepting species of lysine tRNA from rabbit reticulocytes in codon recognition and in haemoglobin biosynthesis in vitro.
    Eur J Biochem. 1971 Dec;24(2):313-20 PMID: 4945501
  10. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.
    Nat New Biol. 1972 Feb 9;235(58):163-7 PMID: 4334193
  11. In vitro synthesis of DNA components of human genes for globins.
    Nat New Biol. 1972 Feb 9;235(58):167-9 PMID: 4334194
  12. Purification of the DNA polymerase of avian myeloblastosis virus.
    Biochim Biophys Acta. 1971 Sep 24;246(3):365-83 PMID: 4334983
  13. Ribonucleic acid directed deoxyribonucleic acid synthesis by the purified deoxyribonucleic acid polymerase of Rous sarcoma virus. Characterization of the enzymatic product.
    Biochemistry. 1972 Jun 6;11(12):2343-51 PMID: 4337615
  14. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  15. Synthesis of DNA from vaccinia messenger RNA templates.
    Nat New Biol. 1972 Aug 2;238(83):139-41 PMID: 4340793
  16. Coding properties of reticulocyte lysine transfer RNA's in hemoglobin synthesis.
    Science. 1972 Sep 29;177(4055):1197-9 PMID: 5057626
  17. Sequence studies of poliovirus RNA. I. Characterization of the 5'-terminus.
    J Mol Biol. 1972 Jul 28;68(3):537-40 PMID: 4341807
  18. Evidence for single copies of globin genes in the mouse genome.
    Nature. 1972 Sep 22;239(5369):219-21 PMID: 4117010
  19. Poly A synthesizing activity in a constitutive subunit of RNA polymerase.
    Nat New Biol. 1972 Nov 8;240(97):35-8 PMID: 4564498
  20. Transcription of ribonucleic acid by the ribonucleic acid directed deoxyribonucleic acid polymerase of Rous sarcoma virus and deoxyribonucleic acid polymerase I of Escherichia coli.
    Biochemistry. 1973 Jan 30;12(3):460-7 PMID: 4345804
  21. The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. 1. Nucleotide sequence analysis of T 1 and pancreatic ribonuclease digestion products.
    Biochimie. 1972;54(7):853-900 PMID: 4569972
  22. In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumour.
    Nat New Biol. 1973 Feb 7;241(110):174-6 PMID: 4121679
  23. Reiteration frequency of duck haemoglobin genes.
    Nat New Biol. 1973 Feb 14;241(111):204-7 PMID: 4512585
  24. Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .
    J Virol. 1973 May;11(5):682-93 PMID: 4350713
  25. Enzymatic synthesis of DNA complementary to purified 14S messenger RNA of immunoglobulin light chain.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):693-6 PMID: 4123688
  26. Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1122-6 PMID: 4123930
  27. Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1809-13 PMID: 4124305
  28. Decreased globin messenger RNA in thalassemia detected by molecular hybridization.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1886-90 PMID: 4124307
  29. Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2029-32 PMID: 4516203
  30. Purification and sequence of messenger RNA for immunoglobulin light chains.
    Nat New Biol. 1973 Aug 22;244(138):236-40 PMID: 4516600
  31. Appendix to studies on kinetoplast DNA: use of nucleic acid fingerprints to estimate the complexity of minicircle DNA.
    Biochim Biophys Acta. 1973 Sep 7;319(3):277-80 PMID: 4747082
  32. Tissue-specific transcription of the globin gene in isolated chromatin.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3440-2 PMID: 4587253
  33. Conditions for using DNA polymerase I as an RNA-dependent DNA polymerase.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1035-9 PMID: 4133845
  34. Nucleotide sequences of human globin messenger RNA.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2300-4 PMID: 4135409
  35. Hydrolysis of polyadenylic acid by pancreatic ribonuclease.
    J Biol Chem. 1960 Aug;235:2393-8 PMID: 13798248
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3502
Published
1974-09-00
Pages
3502-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433802
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3502 PMC Full Text View journal details
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