Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .

Journal of virology ·Vol. 11 ·No. 5 ·1973-05-00 ·Pages 682-93

Zain BS, Dhar R, Weissman SM, Lebowitz P, Lewis AM

Abstract

The DNA of simian virus 40 (SV40) was transcribed into RNA by Escherichia coli RNA polymerase at 18 to 24 C after synchronization of the initiation of RNA synthesis. After a brief synthetic period the RNA product contained relatively large amounts of sequences derived from a limited segment of SV40 DNA. The source for this pulse-labeled RNA was found to be a portion of the segment of SV40 DNA included within the nondefective adenovirus (Ad)-SV40 hybrid viruses, Ad2(+)ND(1) and Ad2(+)ND(3). After synthesis with [gamma-(32)P] ATP, Ad2(+)ND(1) and Ad2(+)ND(3) DNA transcripts contained an initial sequence missing from Ad2 transcripts. This sequence was identified as an initiation sequence for polymerase transcription of the SV40 DNA. Thus, there is a preferred site for initiation of in vitro transcription on the segment of SV40 DNA common to the nondefective Ad2(+)ND(1) and Ad2(+)ND(3) hybrid viruses.

MeSH Terms

Adenoviridae/analysis Autoradiography Base Sequence Centrifugation, Density Gradient Chromatography, Gel Chromatography, Thin Layer DNA, Viral/analysis,isolation & purification DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Hybridization, Genetic Nucleic Acid Hybridization Oligonucleotides/analysis Phosphorus Isotopes RNA, Viral/analysis,biosynthesis,isolation & purification Ribonucleases Simian virus 40/analysis Transcription, Genetic

Chemicals

DNA, Viral Oligonucleotides Phosphorus Isotopes RNA, Viral DNA-Directed RNA Polymerases Ribonucleases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Zain B S

Dhar R

Weissman S M

Lebowitz P

Lewis A M

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23 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.5.682-693.1973

Published

1973-05-00

Pages

682-93

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355164

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.5.682-693.1973 PMC Full Text View journal details

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Preferred site for initiation of RNA transcription by Escherichia coli RNA polymerase within the simian virus 40 DNA segment of the nondefective adenovirus-simian virus 40 hybrid viruses Ad2 + ND 1 and Ad2 + ND 3 .

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