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PMID: 847466 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequences from a rabbit alpha globin gene inserted in a chimeric plasmid.

Science (New York, N.Y.) ·Vol. 196 ·No. 4286 ·1977-04-08 ·Pages 192-5

Liu AY, Paddock GV, Heindell HC, Salser W

Abstract

Rabbit alpha globin gene copies have been made, using reverse transcriptase and DNA polymerase I, and cloned in bacterial plasmids. Plasmid pHb72 has been shown to contain the alpha gene sequence by restriction enzyme analysis and nucleotide sequencing studies, and therefore has been approved for propagation under P2 plus EK1 conditions by the National REcombinant DNA Committee.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Recombinant/analysis,metabolism Extrachromosomal Inheritance Genes Globins Plasmids RNA, Messenger/analysis Research Design/standards
Chemicals
DNA, Recombinant RNA, Messenger Globins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liu A Y
Paddock G V
Heindell H C
Salser W
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
Published
1977-04-08
Pages
192-5
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Databases
GENBANK
M10846
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