Home LiteratureArticle Details

PMID: 6934534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of a human histocompatibility antigen cDNA fragment.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 6081-5

Ploegh HL, Orr HT, Strominger JL

Abstract

A clone (pHLA-1) containing HLA-specific cDNA was constructed by reverse transcription of partially purified HLA mRNA from the human lymphoblastoid cell line LKT. The identity of pHLA-1 was established by its ability to hybridize to HLA heavy chain mRNA and by nucleotide sequence analysis. The pHLA-1 cDNA insert (approximately 525 base pairs) corresponds to the COOH-terminal 46 amino acids of an HLA-A, -B, or -C antigen (15 residues from the hydrophobic region and the remainder from the COOH-termial hydrophilic region), together with a portion of the 3' untranslated region of the mRNA.

MeSH Terms

Base Sequence Cloning, Molecular/methods Codon DNA, Recombinant Genes HLA Antigens/genetics Humans RNA, Messenger/genetics

Chemicals

Codon DNA, Recombinant HLA Antigens RNA, Messenger

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ploegh H L

Orr H T

Strominger J L

References (26)

26 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Determination of the 3' terminal nucleotide of DNA fragments.
Nucleic Acids Res. 1975 Mar;2(3):319-25 PMID: 165468
Isolation and N-terminal amino acid sequence of membrane-bound human HLA-A and HLA-B antigens.
Nature. 1976 May 20;261(5557):200-5 PMID: 1272392
Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region.
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2481-5 PMID: 781677
The major histocompatibility complex--genetics and biology. (First of three parts).
N Engl J Med. 1976 Oct 7;295(15):806-13 PMID: 60701
3' non-coding region sequences in eukaryotic messenger RNA.
Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
HLA membrane antigens: sequencing by intrinsic radioactivity.
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4487-91 PMID: 794879
Assignment of the major histocompatibility complex to a region of the short arm of human chromosome 6.
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1147-51 PMID: 265561
Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
Sequence of the COOH-terminal hydrophilic region of histocompatibility antigens HLA-A2 and HLA-B7.
J Biol Chem. 1978 Aug 10;253(15):5319-24 PMID: 670198
Purification of vitellogenin mRNA and serum albumin mRNA from avian liver by preparative gel electrophoresis.
Eur J Biochem. 1978 Aug 15;89(1):67-79 PMID: 699917
The major histocompatibility complex of the mouse.
Science. 1979 Feb 9;203(4380):516-21 PMID: 104386
Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens.
Proc Natl Acad Sci U S A. 1979 May;76(5):2273-7 PMID: 287067
Selective labelling of the hydrophobic segments of intrinsic membrane proteins with a lipophilic photogenerated carbene.
Nature. 1979 Aug 30;280(5725):841-3 PMID: 471056
The heavy chain of human histocompatibility antigen HLA-B7 contains an immunoglobulin-like region.
Nature. 1979 Nov 15;282(5736):266-70 PMID: 388231
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4927-31 PMID: 291909
Comparison of amino acid sequences of two human histocompatibility antigens, HLA-A2 and HLA-B7: location of putative alloantigenic sites.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4395-9 PMID: 92029
Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. 2. Sequence determination and search for homologies.
Biochemistry. 1979 Dec 11;18(25):5711-20 PMID: 518865
Assembly and maturation of HLA-A and HLA-B antigens in vivo.
Cell. 1979 Dec;18(4):979-91 PMID: 93026
Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.
Proc Natl Acad Sci U S A. 1980 Feb;77(2):960-4 PMID: 6928693
Addition of homopolymers to the 3'-ends of duplex DNA with terminal transferase.
Methods Enzymol. 1979;68:41-50 PMID: 542126
Maximizing gene expression on a plasmid using recombination in vitro.
Methods Enzymol. 1979;68:473-82 PMID: 232222
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.
Gene. 1980 Jul;10(2):177-83 PMID: 6248422

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.10.6081

Published

1980-10-00

Pages

6081-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350217

Subset

Grants

NIAID NIH HHS · AI-10736 · United States

NIADDK NIH HHS · AM-13230 · United States

Databases

GENBANK

J00192

External Links

PubMed source DOI: 10.1073/pnas.77.10.6081 PMC Full Text View journal details

Analysis Services

Molecular cloning of a human histocompatibility antigen cDNA fragment.

Contact

WeChat Official Account