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PMID: 6928693 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Construction of recombinant plasmids containing rat muscle actin and myosin light chain DNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 2 ·1980-02-00 ·Pages 960-4

Katcoff D, Nudel U, Zevin-Sonkin D, Carmon Y, Shani M, Lehrach H, Frischauf AM, Yaffe D

Abstract

The construction and partial characterization of recombinant bacterial plasmids carrying DNA sequences that hybridize with rat skeletal muscle actin and a myosin light chain mRNA is described. DNA of one clone hybridizes specifically with the muscle-specific alpha-actin mRNA. Three plasmid clones contain DNA inserts that hybridize with muscle as well as with nonmuscle actin mRNA. A fifth plasmid contains sequences complementary to mRNA coding for myosin light chain 2. DNA of this plasmid hybridizes specifically with RNA extracted from muscle and differentiated muscle cultures but not with RNA extracted from proliferating mononucleated myogenic cells.

MeSH Terms
Actins/genetics Animals DNA, Recombinant Genes Molecular Weight Muscles Myosins/genetics Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics Rats
Chemicals
Actins DNA, Recombinant RNA, Messenger Myosins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Katcoff D
Nudel U
Zevin-Sonkin D
Carmon Y
Shani M
Lehrach H
Frischauf A M
Yaffe D
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.2.960
Published
1980-02-00
Pages
960-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348403
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.2.960 PMC Full Text View journal details
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