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PMID: 6298772 Published · ppublish English Journal Article

Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 3 ·1983-02-00 ·Pages 731-5

Devare SG, Reddy EP, Law JD, Robbins KC, Aaronson SA

Abstract

The complete nucleotide sequence of the proviral genome of simian sarcoma virus (SSV), an acute transforming retrovirus of primate origin, has been determined. Like other transforming viruses, SSV contains sequences derived from its helper virus, simian sarcoma-associated virus (SSAV), and a cell-derived (v-sis) insertion sequence. By comparison with the sequence of Moloney murine leukemia virus, it was possible to precisely localize and define sequences contributed by SSAV during the generation of SSV. Comparative sequence analysis of SSV and SSAV showed that SSAV provides regulatory sequences for initiation and termination of transcription of the SSV transforming gene. Moreover, coding sequences for the putative protein product of this gene appear to initiate from the amino terminus of the SSAV env gene. Antibodies to synthetic peptides derived from the carboxy and amino termini of the putative protein predicted by the open reading frame identified within v-sis specifically detect a Mr 28,000 protein, p28sis, in SSV-transformed cells. These and other findings confirm the predicted amino acid sequence of this protein and localize it to the coding region of the SSV transforming gene.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Transformation, Viral Genes Genes, Viral Oncogenes Protein Biosynthesis Retroviridae/genetics Sarcoma Virus, Woolly Monkey/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Devare S G
Reddy E P
Law J D
Robbins K C
Aaronson S A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.3.731
Published
1983-02-00
Pages
731-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393453
Subset
IM
Databases
GENBANK
J02394
External Links
PubMed source DOI: 10.1073/pnas.80.3.731 PMC Full Text View journal details
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