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PMID: 205879 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Protein kinase activity associated with the avian sarcoma virus src gene product.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 4 ·1978-04-00 ·Pages 2021-4

Collett MS, Erikson RL

Abstract

Incorporation of phosphorus from [gamma-32P]ATP into protein was catalyzed by specific immunoprecipitates from avian sarcoma virus (ASV)-transformed avian and mammalian cells. This incorporation was observed only when antiserum from tumor-bearing rabbits able to specifically precipitate the ASV sarcoma gene product, p60src, was used to immunoprecipitate antigens from transformed cell lysates. Immunoprecipitates of extracts from normal cells or cells infected with a transformation-defective ASV mutant showed no activity in this assay, nor did any immune complexes formed with normal rabbit serum and any of the cell extracts tested. The expression of the protein kinase activity (ATP:protein phosphotransferase, EC 2.7.1.37) was growth temperature-dependent in cells infected with an ASV mutant temperature-sensitive for the transformation. These results on an enzymatic activity associated with the ASV transforming protein are discussed in terms of protein phosphorylation as a mechanism for viral transformation.

MeSH Terms
Alpharetrovirus/enzymology,genetics Antigen-Antibody Reactions Cell Transformation, Viral Genes, Viral Mutation Protein Kinases/genetics,immunology Temperature
Chemicals
Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collett M S
Erikson R L
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.4.2021
Published
1978-04-00
Pages
2021-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392475
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.4.2021 PMC Full Text View journal details
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