Nucleotide sequence of the transforming gene of simian sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 10 ·1982-05-00 ·Pages 3179-82

Devare SG, Reddy EP, Robbins KC, Andersen PR, Tronick SR, Aaronson SA

Abstract

The sequence of the transforming region of simian sarcoma virus (SSV) has been determined by using molecularly cloned viral DNA. This region encompassed the 1.0-kilobase pair woolly monkey cell-derived insertion sequence, v-sis, and flanking simian sarcoma-associated viral (SSAV) sequences. A 675-nucleotide-long open reading frame commenced 19 nucleotides within the SSAV sequences to the left of the v-sis helper viral junction and terminated within v-sis itself. Possible promoter and acceptor splice signals were detected in helper viral sequences upstream from this open reading frame, and potential polyadenylylation sites were identified downstream both within v-sis and in helper viral sequences beyond v-sis. The recombinational event that led to the generation of SSV occurred in the middle of two functional codons, indicating that SSAV provided the regulatory elements for transcription as well as the initiation codon for translation of SSV cell-derived transforming sequences.

MeSH Terms

Cell Transformation, Viral DNA, Viral/genetics Gene Expression Regulation Genes Genes, Regulator Genes, Viral Helper Viruses/genetics RNA, Viral Retroviridae/genetics Sarcoma Virus, Woolly Monkey/genetics Transcription, Genetic Viral Proteins/genetics

Chemicals

DNA, Viral RNA, Viral Viral Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Devare S G

Reddy E P

Robbins K C

Andersen P R

Tronick S R

Aaronson S A

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27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.10.3179

Published

1982-05-00

Pages

3179-82

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC346378

Subset

Databases

GENBANK

J02396, J02397

External Links

PubMed source DOI: 10.1073/pnas.79.10.3179 PMC Full Text View journal details

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Nucleotide sequence of the transforming gene of simian sarcoma virus.

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