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PMID: 2987792 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequences of murine intracisternal A-particle gene LTRs have extensive variability within the R region.

Nucleic acids research ·Vol. 13 ·No. 1 ·1985-01-11 ·Pages 289-302

Christy RJ, Brown AR, Gourlie BB, Huang RC

Abstract

Nucleotide sequences of the long terminal repeats (LTRs) of four murine intracisternal A-particle (IAP) genes IAP62, 19, 81 and 14 were determined. Each IAP LTR contains three sequence domains, 5'-U3-R-U5-3', and each is bound by 4 bp imperfect inverted repeats. The transcriptional regulatory sequences, CAAT and TATA, as well as the enhancer core sequence GTGGTAA are conserved and precisely positioned within the U3 region. In the R region, the sequence AATAAA is located twenty base pairs preceding the dinucleotide CA, the polyadenylation site. In IAP19 and IAP81, the 5' and 3' LTRs are flanked by a six nucleotide direct repeat of cellular sequences representing the possible integration sites for these IAP proviruses. Both the size and sequences of different IAP LTRs vary considerably, with the majority of the variation localized within the R regions. The size of R varies from 66 bp in IAP14 to 222 bp in IAP62; in contrast, the U3 and U5 regions are all similar in size. These extra sequences within the R region of large LTRs consist of several unusual directly repeating sequences which account for this variability.

MeSH Terms
Animals Base Sequence Cloning, Molecular Cricetinae DNA/genetics DNA Transposable Elements DNA, Viral/genetics Genes Genes, Viral Inclusion Bodies, Viral/metabolism Mesocricetus Mice Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Viral DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Christy R J
Brown A R
Gourlie B B
Huang R C
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.1.289
Published
1985-01-11
Pages
289-302
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340991
Subset
IM
Grants
NIA NIH HHS · 5R01AG04350 · United States
NIA NIH HHS · 5T32AG00069 · United States
Databases
GENBANK
X01705, X01706, X01707, X01708, X01709
External Links
PubMed source DOI: 10.1093/nar/13.1.289 PMC Full Text View journal details
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