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PMID: 19555292 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

Advances in light microscopy for neuroscience.

Annual review of neuroscience ·Vol. 32 ·2009-00-00 ·Pages 435-506

Wilt BA, Burns LD, Wei Ho ET, Ghosh KK, Mukamel EA, Schnitzer MJ

Abstract

Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer than the optical diffraction limit. Other progress permits functional imaging at faster speeds, at greater depths in brain tissue, and over larger tissue volumes than previously possible. Portable, miniaturized fluorescence microscopes now allow brain imaging in freely behaving mice. Complementary progress on animal preparations has enabled imaging in head-restrained behaving animals, as well as time-lapse microscopy studies in the brains of live subjects. Mouse genetic approaches permit mosaic and inducible fluorescence-labeling strategies, whereas intrinsic contrast mechanisms allow in vivo imaging of animals and humans without use of exogenous markers. This review surveys such advances and highlights emerging capabilities of particular interest to neuroscientists.

MeSH Terms
Animals Humans Image Cytometry/instrumentation,methods,trends Mice Mice, Transgenic Microscopy/instrumentation,methods,trends Microscopy, Confocal/instrumentation,methods,trends Microscopy, Fluorescence/instrumentation,methods,trends Molecular Biology/instrumentation,methods,trends Nervous System/cytology Neurons/cytology,physiology Neurosciences/instrumentation,methods,trends
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wilt Brian A
James H. Clark Center and Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, USA.
Burns Laurie D
Wei Ho Eric Tatt
Ghosh Kunal K
Mukamel Eran A
Schnitzer Mark J
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Article Info
Journal
Annual review of neuroscience
Abbr.
Annu Rev Neurosci
ISSN
1545-4126
DOI
10.1146/annurev.neuro.051508.135540
Published
2009-00-00
Pages
435-506
Language
English
Region
United States
NLM ID
7804039
PMCID
PMC2820375
Subset
IM
Grants
NINDS NIH HHS · R01 NS050533-01 · United States
NIDA NIH HHS · R21 DA017895 · United States
NIH HHS · DP1 OD003560 · United States
NINDS NIH HHS · R01 NS050533 · United States
NIDA NIH HHS · R21 DA017895-01 · United States
NIH HHS · DP1 OD003560-01 · United States
External Links
PubMed source DOI: 10.1146/annurev.neuro.051508.135540 PMC Full Text View journal details
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