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PMID: 19259228 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

In vivo functional imaging of intrinsic scattering changes in the human retina with high-speed ultrahigh resolution OCT.

Optics express ·Vol. 17 ·No. 5 ·2009-03-02 ·Pages 3861-77

Srinivasan VJ, Chen Y, Duker JS, Fujimoto JG

Abstract

Non-invasive methods of probing retinal function are of interest for the early detection of retinal disease. While retinal function is traditionally directly measured with the electroretinogram (ERG), recently functional optical imaging of the retina has been demonstrated. In this manuscript, stimulus-induced, intrinsic optical scattering changes in the human retina are measured in vivo with high-speed, ultrahigh resolution optical coherence tomography (OCT) operating at 50,000 axial scans per second and ~3.3 micron axial resolution. A stimulus and measurement protocol that enables measurement of functional OCT retinal signals is described. OCT signal changes in the photoreceptors are demonstrated. Two distinct responses having different temporal and spatial properties are reported. These results are discussed in the context of optical intrinsic signals measured previously in the retina by fundus imaging and scanning laser ophthalmoscopy. Finally, challenges associated with in vivo functional retinal imaging in human subjects are discussed.

MeSH Terms
Adult Biometry Electroretinography Equipment Design Humans Male Models, Theoretical Optical Phenomena Photic Stimulation Photoreceptor Cells, Vertebrate/physiology,radiation effects Retina/physiology,radiation effects Retinal Diseases/diagnosis,physiopathology Scattering, Radiation Tomography, Optical Coherence/instrumentation,methods
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Srinivasan V J
Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Chen Y
Duker J S
Fujimoto J G
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Article Info
Journal
Optics express
Abbr.
Opt Express
ISSN
1094-4087
DOI
10.1364/oe.17.003861
Published
2009-03-02
Pages
3861-77
Language
English
Region
United States
NLM ID
101137103
PMCID
PMC2846082
Subset
IM
Grants
NEI NIH HHS · R01 EY011289-23 · United States
NEI NIH HHS · P30-20EY008098 · United States
NEI NIH HHS · R01-EY11289-24 · United States
NEI NIH HHS · R01 EY011289-26 · United States
NEI NIH HHS · R01 EY013178 · United States
NEI NIH HHS · R01 EY011289 · United States
NEI NIH HHS · R01-EY13178-07 · United States
NEI NIH HHS · P30 EY008098 · United States
External Links
PubMed source DOI: 10.1364/oe.17.003861 PMC Full Text View journal details
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