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PMID: 18192022 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy.

Journal of neuroscience methods ·Vol. 169 ·No. 1 ·2008-03-30 ·Pages 1-7

Davalos D, Lee JK, Smith WB, Brinkman B, Ellisman MH, Zheng B, Akassoglou K

Abstract

In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful tool in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease.

MeSH Terms
Acepromazine/pharmacology Anesthesia/methods Anesthetics/pharmacology Animals Axons/physiology,ultrastructure Capillaries/cytology,physiology Cell Movement/physiology Green Fluorescent Proteins/genetics Image Processing, Computer-Assisted/methods Ketamine/pharmacology Laminectomy/methods Mice Mice, Transgenic Microcirculation/cytology,physiology Microglia/cytology,physiology Microscopy, Confocal/methods Neurosurgical Procedures/methods Respiratory Physiological Phenomena/drug effects Spinal Cord/blood supply,cytology Staining and Labeling/methods Stereotaxic Techniques/instrumentation Urethane/pharmacology Xylazine/pharmacology
Chemicals
Anesthetics Green Fluorescent Proteins Xylazine Urethane Acepromazine Ketamine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Davalos Dimitrios
Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. davalos@ucsd.edu
Lee Jae K
Smith W Bryan
Brinkman Brendan
Ellisman Mark H
Zheng Binhai
Akassoglou Katerina
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Article Info
Journal
Journal of neuroscience methods
Abbr.
J Neurosci Methods
ISSN
0165-0270
DOI
10.1016/j.jneumeth.2007.11.011
Email
davalos@ucsd.edu
Published
2008-03-30
Epub
2007-00-28
Pages
1-7
Language
English
Region
Netherlands
NLM ID
7905558
PMCID
PMC2647134
Subset
IM
Grants
NCRR NIH HHS · RR04050 · United States
NINDS NIH HHS · P30 NS047101 · United States
NINDS NIH HHS · NS052189 · United States
NINDS NIH HHS · F32 NS056697-02 · United States
NINDS NIH HHS · R01 NS054734 · United States
NCRR NIH HHS · P41 RR004050 · United States
NINDS NIH HHS · F32 NS056697 · United States
NINDS NIH HHS · R01 NS052189 · United States
NINDS NIH HHS · R01 NS051470 · United States
NINDS NIH HHS · NS051470 · United States
External Links
PubMed source DOI: 10.1016/j.jneumeth.2007.11.011 PMC Full Text View journal details
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