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PMID: 17267604 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Olfactory neurons expressing transient receptor potential channel M5 (TRPM5) are involved in sensing semiochemicals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 7 ·2007-02-13 ·Pages 2471-6

Lin W, Margolskee R, Donnert G, Hell SW, Restrepo D

Abstract

Olfactory sensory neurons (OSNs) in the main olfactory epithelium respond to environmental odorants. Recent studies reveal that these OSNs also respond to semiochemicals such as pheromones and that main olfactory input modulates animal reproduction, but the transduction mechanism for these chemosignals is not fully understood. Previously, we determined that responses to putative pheromones in the main olfactory system were reduced but not eliminated in mice defective for the canonical cAMP transduction pathway, and we suggested, on the basis of pharmacology, an involvement of phospholipase C. In the present study, we find that a downstream signaling component of the phospholipase C pathway, the transient receptor potential channel M5 (TRPM5), is coexpressed with the cyclic nucleotide-gated channel subunit A2 in a subset of mature OSNs. These neurons project axons primarily to the ventral olfactory bulb, where information from urine and other socially relevant signals is processed. We find that these chemosignals activate a subset of glomeruli targeted by TRPM5-expressing OSNs. Our data indicate that TRPM5-expressing OSNs that project axons to glomeruli in the ventral area of the main olfactory bulb are involved in processing of information from semiochemicals.

MeSH Terms
Animals Axons Cyclic Nucleotide-Gated Cation Channels Ion Channels/analysis Mice Mice, Transgenic Olfactory Bulb/physiology Olfactory Receptor Neurons/chemistry,physiology Pheromones/physiology Signal Transduction TRPM Cation Channels/analysis,physiology Transient Receptor Potential Channels Type C Phospholipases/metabolism
Chemicals
Cnga2 protein, mouse Cyclic Nucleotide-Gated Cation Channels Ion Channels Pheromones TRPM Cation Channels Transient Receptor Potential Channels Trpm5 protein, mouse Type C Phospholipases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lin Weihong
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD 21250, USA.
Margolskee Robert
Donnert Gerald
Hell Stefan W
Restrepo Diego
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0610201104
Published
2007-02-13
Epub
2007-00-31
Pages
2471-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1892929
Subset
IM
Grants
NIDCD NIH HHS · P30 DC004657 · United States
NIDCD NIH HHS · DC006828 · United States
NIDCD NIH HHS · R03 DC006828 · United States
NIDCD NIH HHS · DC004657 · United States
NIDCD NIH HHS · R01 DC003055 · United States
NIDCD NIH HHS · DC006070 · United States
NIDCD NIH HHS · R01 DC003155 · United States
NIDCD NIH HHS · R03 DC006828-02 · United States
NIDCD NIH HHS · DC03155 · United States
NIDCD NIH HHS · DC00566 · United States
NIDCD NIH HHS · R01 DC000566 · United States
NIDCD NIH HHS · DC03055 · United States
NIDCD NIH HHS · R01 DC006070 · United States
NIDCD NIH HHS · R01 DC009269 · United States
External Links
PubMed source DOI: 10.1073/pnas.0610201104 PMC Full Text View journal details
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