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PMID: 7112109 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.

Science (New York, N.Y.) ·Vol. 217 ·No. 4563 ·1982-09-03 ·Pages 953-5

Maranto AR

Abstract

Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.

MeSH Terms

3,3'-Diaminobenzidine Animals Astacoidea Ganglia/physiology,ultrastructure In Vitro Techniques Isoquinolines Leeches Light Microscopy, Electron/methods Neurons/physiology,ultrastructure Synapses/ultrastructure

Chemicals

Isoquinolines 3,3'-Diaminobenzidine lucifer yellow

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Maranto A R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.7112109

Published

1982-09-03

Pages

953-5

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.7112109 View journal details

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Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy.

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