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PMID: 15128753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.

Journal of neurophysiology ·Vol. 92 ·No. 5 ·2004-11-00 ·Pages 3121-33

Jung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ

Abstract

One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells in vivo. Both one- and two-photon fluorescence microendoscopy are based on compound gradient refractive index (GRIN) lenses that are 350-1,000 microm in diameter and provide micron-scale resolution. One-photon microendoscopy allows full-frame images to be viewed by eye or with a camera, and is well suited to fast frame-rate imaging. Two-photon microendoscopy is a laser-scanning modality that provides optical sectioning deep within tissue. Using in vivo microendoscopy we acquired video-rate movies of thalamic and CA1 hippocampal red blood cell dynamics and still-frame images of CA1 neurons and dendrites in anesthetized rats and mice. Microendoscopy will help meet the growing demand for in vivo cellular imaging created by the rapid emergence of new synthetic and genetically encoded fluorophores that can be used to label specific brain areas or cell classes.

MeSH Terms
Animals Dendrites/ultrastructure Endoscopy Erythrocytes/cytology Female Fluorescent Dyes Mammals Mice Microscopy, Fluorescence Neurons/cytology,physiology Photons Rats Rats, Sprague-Dawley Sensitivity and Specificity Video Recording
Chemicals
Fluorescent Dyes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jung Juergen C
Department of Biological Sciences, Stanford University, Stanford, CA 94305-5435, USA.
Mehta Amit D
Aksay Emre
Stepnoski Raymond
Schnitzer Mark J
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Article Info
Journal
Journal of neurophysiology
Abbr.
J Neurophysiol
ISSN
0022-3077
DOI
10.1152/jn.00234.2004
Published
2004-11-00
Epub
2004-00-05
Pages
3121-33
Language
English
Region
United States
NLM ID
0375404
PMCID
PMC2826362
Subset
IM
Grants
NIDA NIH HHS · R21 DA017895 · United States
NIDA NIH HHS · R21 DA017895-01 · United States
NIDA NIH HHS · 1R21DA-017895-01 · United States
External Links
PubMed source DOI: 10.1152/jn.00234.2004 PMC Full Text View journal details
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