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PMID: 17988631 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Axonal motility and its modulation by activity are branch-type specific in the intact adult cerebellum.

Neuron ·Vol. 56 ·No. 3 ·2007-11-08 ·Pages 472-87

Nishiyama H, Fukaya M, Watanabe M, Linden DJ

Abstract

We performed two-photon in vivo imaging of cerebellar climbing fibers (CFs; the terminal arbor of olivocerebellar axons) in adult mice. CF ascending branches innervate Purkinje cells while CF transverse branches show a near complete failure to form conventional synapses. Time-lapse imaging over hours or days revealed that ascending branches were very stable. However, transverse branches were highly dynamic, exhibiting rapid elongation and retraction and varicosity turnover. Thus, different branches of the same axon, with different innervation patterns, display branch type-specific motility in the adult cerebellum. Furthermore, dynamic changes in transverse branch length were almost completely suppressed by pharmacological stimulation of olivary firing.

MeSH Terms
Action Potentials/physiology Animals Axons/physiology,ultrastructure Cell Movement/physiology Cell Shape/physiology Cerebellar Cortex/cytology,physiology Fluorescent Dyes Growth Cones/physiology,ultrastructure Mice Mice, Inbred C57BL Mice, Transgenic Neural Pathways/cytology,physiology Neuronal Plasticity/physiology Olivary Nucleus/cytology,physiology Organ Culture Techniques Organic Chemicals Presynaptic Terminals/physiology,ultrastructure Synaptic Transmission/physiology Time Factors
Chemicals
Alexa594 Fluorescent Dyes Organic Chemicals
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nishiyama Hiroshi
Department of Neuroscience, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, USA.
Fukaya Masahiro
Watanabe Masahiko
Linden David J
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/j.neuron.2007.09.010
Published
2007-11-08
Pages
472-87
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2098835
Subset
IM
Grants
NIMH NIH HHS · R37 MH51106 · United States
NIMH NIH HHS · R37 MH051106-15 · United States
NIMH NIH HHS · P50 MH068830-010003 · United States
NIMH NIH HHS · R37 MH051106 · United States
NIMH NIH HHS · P50 MH068830 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2007.09.010 PMC Full Text View journal details
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